摘要
选择关中盆地马兰黄土为研究对象，采用平板菌落计数法研究了与三氮转化有关的微生物在0～9m剖面上的垂直分布。结果表明，剖面上氨化细菌、硝化细菌、反硝化细菌、固氮菌分别为克土10^6～10^10、10^1～10^3、10^3～10^8、10^3～10^5个。在试验区剖面7m处的土壤中存在着反硝化菌富集区，数量达克土10^8个。对此层位土壤进行了反硝化能力实验，结果表明，马兰黄土反硝化作用最适宜温度为22℃和37℃，碳源的存在可大大提高反硝化速率，但改变碳氮比对反硝化速率影响不大。在15℃、无外界供给碳源、硝酸根初始质量浓度为56mg／L情况下，反硝化菌对硝酸根的降解作用符合方程Y=-2、6828x＋60．979，回归系数R=0．9609。该反硝化菌富集区的存在为研究包气带中三氮转化提供了重要信息。
This work studied the microorganisms distribution in the soil sampled from 0 to 9 m in Malan Loess, Guanzhong Basin, aiming to understand its transfer of nitrate, nitrite and ammonia. The microorganisms were observed to be ammonifying bacteria, nitrifying bacteria, denitrifying bacteria and azotobacter, with a quantity of 10^1- 10^3 , 10^1- 10^3 , 10^3 - 10^8 and 10^3- 10^5 g^ -1 soil, respectively. In the soil at 7 m depth, the quantity of the denitrifying bacteria reached the peak of 10^8 g ^-1soil, and its optimum temperatures for the denitrifying of Malan Loess were found to be 22 ℃ and 37℃ respectively. Denitrifying rate may increase in the presence of carbon sources, but little effect by changing the C/N ratios, and its denitrifying kinetic curves of y=-2. 682 8x＋60. 979 with the regressive coefficient R=0. 960 9 was obtained at 15 ℃ and the absence of foreign carbon sources with 56 mg/L nitrate. This special soil specimen offered the biological treatment basis to the nitrate pollution in saturated zone and ground water.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2006年第6期411-415,共5页
Environmental Pollution & Control
基金
国家重点基础研究发展规划资助项目(No.G1999043606)
关键词
微生物
三氮
反硝化
马兰黄土
关中盆地
Microorganisms Nitrogen Denitrifying Malan loess Guanzhong basin
