摘要
目的探讨凋亡相关蛋白Survivin、Fas在肾母细胞瘤发生发展中的作用,为其预后判断及治疗方案制定提供依据。方法选取30例术前均未化疗的肾母细胞瘤石蜡包埋和15例正常肾组织标本,应用免疫组织化学方法检测Survivin、Fas蛋白在上述标本中的表达。结果Survivin蛋白在肾母细胞瘤中表达率为53.3%,高于正常肾组织0;Fas蛋白在肾母细胞瘤中表达率为36.6%,低于正常肾组织80.0%,二者均有统计学差异(P均<0.05)。Survivin蛋白在预后不良(UH)组及预后良好(FH)组表达率分别为83.3%、45.8%,在晚期及早期病例组中表达率分别为83.3%、33.3%,在有转移组与无转移组之间的表达率分别为87.5%、40.9%,差异均有统计学意义(P均<0.05)。Survivin蛋白阳性患儿2年生存率为69.2%,明显低于阴性表达患儿(100%)(P<0.05)。结论小儿肾母细胞瘤组织中存在Survivin蛋白高表达及Fas蛋白低表达,二者协同参与肾母细胞瘤的发生和发展;Survivin可作为肾母细胞瘤预后的重要指标。
Objective To explore the role of Survivin and Fas in the tumor cells＇ invasion and metastasis and to evaluate whether they can be used as a reliable predictor for diagnosis and a new treatment pattern for therapy. Methods Thirty paraffin-embeded material of nephroblastoma were collected. The control group was composed of 15 specimens of normal kidney tissue acquired from paraturnor region of the material. Immunohistoehernistry methods was used to detect the stain of Survivin and Fas proteins in normal kidney and nephroblastoma specimens, compared them with clinical stage, pathology type and the outcome. Results Positive expression rate of Survivin in tumor specimens and significantly higher than that in control group （53.3 % vs 0, P 〈 0.01 ）, while positive rate of Fas in nephroblastoma was lower than that of control（36.6 % vs 80.0 %, P 〈 0.01 ）. The positive rate of Survivin were 83.3 %, 45.8 % in unfavorable histology （UH） and fauorable histology（FH） groups, and 83.3 %, 33.3 % in later and early clinical stage, respectively. Statistical analysis revealed that expression of Survivin in nephroblastoma was significantly correlated with clinical stage and pathological type, but there was no correlationship between Fas expression and clinical or pathological stage. In addition, the follow - up data suggested that the survival rate in Survivin positive cases be signigicantly lower than that of cases without Survivin expression. Conclusions Both Survivin and Fas participated in the tumorgenesis of nephroblastoma, and Survivin - induced apoptosis inhibition and angiogenesis are significantly associated with the development of nephroblastoma. Expression of Survivin may be an important parameter in the evaluation of malignance and progress of nephroblastoma.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第11期668-669,712,共3页
Journal of Applied Clinical Pediatrics
