摘要
目的:观察病变侧缺血至再灌期亚低温(32～33℃)对局灶脑缺血再灌注后梗死体积、水肿程度、谷氨酸转运体功能及谷氨酸含量的影响。方法:实验于2005-08/11在新乡医学院第一附属医院中心实验室完成。①80只大鼠随机分为假手术组16只、常温脑缺血组32只和亚低温脑缺血组32只。3组再均分为缺血再灌注后2h,1,3,7d4个时间点,假手术组每个时间点4只,另两组每个时间点8只。②采用改良线栓法建立大鼠大脑中动脉缺血再灌注模型,缺血即刻应用采用可调控半导体制冷仪对大鼠病变侧给予亚低温治疗,并持续至再灌期。③采用TTC染色测梗死体积,物理方法测水肿程度,利用脑组织突触膜颗粒对3H-L-谷氨酸摄入量的测定及分光光度法观察皮层谷氨酸转运体功能及高效液相色谱仪测定谷氨酸含量。结果:80只大鼠均进入结果分析。①同常温脑缺血组相比,亚低温脑缺血组梗死体积明显减少(梗死体积减少56.3%～66.0%)。②同常温脑缺血组相比,亚低温脑缺血组水肿程度明显减少(减少2.8%～6.5%)。③同常温脑缺血组相比,亚低温脑缺血组谷氨酸转运体功能增强(P<0.05)。④同常温脑缺血组相比,亚低温脑缺血组谷氨酸含量降低(P<0.05)。结论:病变侧亚低温能显著减小脑梗死体积和脑水肿,其机制可能为通过谷氨酸转运体降低谷氨酸的含量。
AIM： To observe the effects of ischemia in affected side and mild hypothermia （32-33 ℃） of reperfusion period on infarct volume, eXtent of cerebral edema, function of glutamate transporter and content of glutamate in rats with focal cerebral ischemic reperfusion.
METHODS： The experiment was conducted in the Department of Neurology, First Affiliated Hospital of Xinxiang Medical College from August to November 2005. （1）A total of 80 rats were randomly divided into sham-operation group （n=16）, normothermia cerebral ischemia group （n=32） and mild hypothermia group （n=32）, which were subdivided according to four different time-peints of pest-ischemic reperfusion： 2^nd hour, 1^st day, 3^nd day and 7^th day. There were 4 rats in each time-peint of the sham-operation group and 8 rats in each time-peint of the other two groups. （2）Rat models with middle cerebral artery （MCA） ischemic reperfusion were established with modified suture occlusion method. Affected sides of rats in ischemic moment were treated with mild hypothermia by regulated semiconductor cooling meter and the treatment lasted to reperfusion.（3）Infarct volume was measured with TTC stain and extent of cerebral edema was detected with physical method. Synaptic membrane （SM） gradule in brain tissues was used to determine the intake of ^3H-L-glutamate and spectrephotometry was adopted in observing the function of glutamate transporter; High performanee liquid chromatogram （HPLC） was used in detecting the content of glutamate.
RESULTS： A total of 80 rats were involved in the analysis of results.（1） Compared with normothermia cerebral ischemic group, infarct volume in mild hypethemia cerebral ischemia group significantly decreased （with the deduction of 563%-66.0% ）. （2）Compared with normothermia cerebral ischemia/group, extent of cerebral edema was obviously decreased in the mild hypethermia group （decreased 2.8%-6.5%）： （3）Compared with normothermia ischemia reperfusion group, function of glutamate transporter in mild hypothermia group increased （P 〈 0.05）. （4） Compared with normothermia ischemic reperfusion group, content of glutamate in the mild hypothermia group decreased （P 〈 0.05）.
CONCLUSION： Mild hypothermia in affected side can markedly reduce the infarct volume and extent of cerebral edema, which may reduce the content of glutamate by reinforcing functions of glutamate transporter.
出处
《中国临床康复》
CSCD
北大核心
2006年第28期61-63,共3页
Chinese Journal of Clinical Rehabilitation
