摘要
目的:探讨慢性不可预见性应激抑郁模型大鼠脑内去甲肾上腺素转运体(NET)基因表达的变化。方法:40只SD雄性大鼠随机分为正常对照组和抑郁模型组。在建立慢性不可预见性应激抑郁模型后,应用动物行为分析系统记录大鼠自主活动的变化,用逆转录_聚合酶链反应法测定NET mRNA的含量。结果:实验后第21 d,抑郁模型组的活动时间、总路程明显减少(P<0.05),休息时间明显延长(P<0.05),脑桥NET基因表达明显下降(P<0.05)。结论:抑郁模型大鼠脑桥NET基因表达下降可能与抑郁障碍发病有关。
Objective： To investigate the expression change of brain norepinephrine transporter（NET）mRNA in rats treated with chronic unpredicted mild stress. Methods： Male Sprague-Dawley rats were randomly divided into normal control group and depression model group. Chronic stress depression rat model was established and then the autonomic behavior was measured by animal behavior analysis system. The expression of mRNA coding for NEH＇ was assayed by reverse transcription-polymerase chain reaction. Results： After unpredicted mild stressors, the motive time and distance were significantly decreased（ P 〈 0.05）, and the resting time was significantly prolonged（ P 〈 0.05）, the expression of NET mRNA in pontine tissue was significantly decreased（ P 〈 0.05）in depression model group, compared with normal control group. Conclusion： The decreased expression of NET mRNA in pontine might be associated with the etiology of depressive disorder.
出处
《汕头大学医学院学报》
2006年第2期82-84,90,共4页
Journal of Shantou University Medical College
基金
广东省自然科学基金资助(04020238)
关键词
抑郁障碍
慢性应激
去甲肾上腺素转运体
逆转录-聚合酶链反应法
depressive disorder
chronic stress
norepinephrine transporter
reverse tmnscriptlon-polymerase chain reaction
