摘要
目的探讨促性腺激素释放激素受体(GnRH R)mRNA在正常子宫内膜及子宫内膜腺癌组织中的表达。方法采用原位杂交技术,检测23份增生期正常子宫内膜、30份分泌期正常子宫内膜及30份子宫内膜腺癌组织中GnRH RmRNA的表达。结果正常子宫内膜及子宫内膜腺癌组织中GnRH RmRNA均呈阳性表达,其阳性信号物质位于细胞质。增生期及分泌期正常子宫内膜组织GnRH RmRNA的阳性表达率分别为17%(4/23)和17%(5/30),两者比较,差异无统计学意义(P>0.05);子宫内膜腺癌组织GnRH RmRNA的阳性表达率为40%(12/30),明显高于正常子宫内膜组织[17%(9/53),P<0.05]。结论GnRH可直接作用于子宫内膜产生内分泌作用,癌变的子宫内膜细胞表面GnRH R明显增多。
Objective To investigate the expression of gonadotropin-releasing hormone receptor （GnRH-R） mRNA in the endometrium of normal and endometrial cancer uterus. Methods In situ hybridization was used to detect the expression of GnRH-R mRNA in 23 cases of normal endometrium in hyperplasic phase, 30 cases of normal endometrium in secretory phase, and 30 cases of endometfium from endometrial cancer. Results The results showed that GnRH-R mRNA was expressed in the cytoplasm of endometrium of normal and endometrial cancer uterus. There was no expression in the nuclei. There was no significant difference （P〉0.05 ） in the expression rate between the hyperplasic phase （ 17% ,4/23 ） and the secretory phase （ 17% ,5/30） in normal uterus. The expression rate in endometrial cancer was 40% （ 12/ 30）, which was significantly higher than normal endometrium （P〈0.05 ） . Conclusions The data demonstrates that GnRH may act directly on endometrium. GnRH-R is highly expressed in the endometrium of endometfial cancer.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2006年第5期326-328,共3页
Chinese Journal of Obstetrics and Gynecology
基金
吉林省科学技术厅科技发展计划资助项目(20030432)
