摘要
用胃蛋白酶-纤维素酶的复合酶解法测定苜蓿干草、红三叶草、豆腐渣、啤酒糟、高梁青贮、羊草、雀麦草、羊茅和稻草的有机物降解率(FOM,％)分别为51.16、70.88、82.29、46.92、44.32、29.64、44.88、45.92、30.92。盐酸胃蛋白酶-纤维素酶技术能很好地估测青粗饲料在瘤胃中的降解率(FOM),对于牧草类FOM值的估测价值为R^2=92％以上(r>0.96)。其操作程序为:(1)0.1N HCI-胃蛋白酶(酶量为150IU/0.3g粗料)在40℃下培养24小时;(2)在pH=4.5的柠檬酸盐缓冲液中用纤维素酶(50mg酶量)培养24小时(40℃)。该法评定青粗饲料FOM不必加水解淀粉的步骤。
The pepsin-celluiase technique was a precise and rapid way to assess the organic matter degradability (FOM) of forages,and the regression coeffecience between the nylon bag and the pepsin-celluiase technique was 0.92.The experimental procedures were: (1) pepsin(150 IU/0.3g sample)in 0.1 N HCL for 24h at 40℃),(2) celluiase (50mg) in citrate buffer (pH=4.5) for 24h at 40℃.The FOM of nine forages,lucerne liny,red clover,soybean crude residue,brewer's grain,sorghum silage,Chinese wildrye,smooth brome,reed fescue and rice straw were determined by the pepsin-celluiase technique.The FOM were 51.16,70.88,82.29,46.92,44.32,29.64,44.88,45.92 and 30.92,respectively.
出处
《动物营养学报》
CAS
CSCD
1996年第3期20-24,共5页
CHINESE JOURNAL OF ANIMAL NUTRITION
