摘要
谷胱甘肽Ｓ－转移酶Ｐ１－１在癌变细胞和抗药性肿瘤细胞中表达水平发生变化，提示可以作为恶性转化及肿瘤抗药性的标志物．对大鼠谷胱甘肽Ｓ－转移酶Ｐ１基因上游调控序列的研究发现在－２．５ｋｂ及－２．２ｋｂ各存在一增强子序列ＧＰＥⅠ，ＧＰＥⅡ，－４００ｂＰ存在一沉寂子．ＧＰＥⅠ、沉寂子上均至少结合有３种反式作用因子．人谷胱甘肽Ｓ－转移酶Ｐ１基因上游区域中迄今尚未发现增强子或沉寂子，但却发现了胰岛素及视黄酸的应答序列，在癌变细胞和抗药性的肿瘤细胞中该基因表达的调控机制有别于正常细胞．
Glutathione S-transferase P1-1(GSTP1-1) is expressed at a different level in tumor cell and drug resistant neoplastic cell.It is suggested that GSTP1-1 can serve as a marker for malignant transformation and drug resistance. On the study upstream regulatory sequence of rat glutathione S-transferase P1(rGSTP1) gene, it is found that two enchancing elements named GPE Ⅰ and GPE Ⅱ arelocated in - 2. 5 kb, - 2. 2 kb respectively.A silencer locates at-400 bp. Both of GPE Ⅰand silencer bind at least three kinds of transacting factors. Although any enhancer or silencer has not been found in human glutathione S-transferase PI (hGSTP1 ) geneso far, but insulin and retinoic acid (RA)responsive sequences have been identified.The regulatory mechanism of hGSTP1 gene in tumor cells and antidrug tumor cells is different from normal cells.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1996年第1期42-45,共4页
Progress In Biochemistry and Biophysics
关键词
肿瘤
谷胱甘肽
S-转移酶P1基因
基因结构
glutathione S-transferase P1 gene, gene structure, regulation of geneexpression, tumor
