摘要
采用生长试验、免疫放射分析和定量PCR方法,观察了生长猪血液中类胰岛素生长因子(IGF-I)及其结合蛋白(IGFBP3)数量的变化和IGF-I基因在肝中表达量的变化。结果显示, 每1 kg饲料中添加125-250 mg铜,能够上调IGF-I基因的表达量,显著提高猪的平均日增重,促进生长猪循环血液中IGF-I浓度的增加。试验结果证实,铜是通过促进生长激素-胰岛素样生长因子轴相关因子的合成和分泌来发挥促生长作用的。
The experiment was conducted in order to explore the effect of copper on growth correlation factor from the level of integer and gene expression, such as porcine liver IGF- Ⅰ, in order to elucidate the modulation action of copper on liver and somatotropin axis correlation factor and internal mechanism of growth-promoting of copper. Quantitative changes of gene expression of IGF- Ⅰ and IGFBP in blood were detected by growth promoting experiment, RIA and quantitative PCR. These results indicated that the addition of copper（125-250 mg/kg） in food improved the IGF- Ⅰgene expression, significantly increased the average body weight gain. These results indicated that copper stimulates growth by the synthesis and secretion of some factors of axis.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第6期497-501,共5页
Chinese Veterinary Science
基金
国家自然科学基金资助项目(39970567)
关键词
铜
猪
肝
类胰岛素生长因子
基因表达
copper
porcine
liver
insulin-like growth factor
gene expression
