摘要
目的 建立家猪胸腰段脊髓火器贯通伤模型和改良Allen打击伤后全瘫模型,观察伤后胶质纤维酸性蛋白（GFAP）的早期表达.方法 实验动物随机分为3组：火器贯通伤组9只,在全麻状态下制作胸腰段（T13～L2）脊髓火器伤模型;打击伤组9只,L1节段脊髓行改良Allen打击;对照组2只.伤后不同时间点（伤后1、3、6 h）和不同节段（伤点、近伤点、中伤点及远伤点）取材,进行GFAP的免疫组化染色.结果 脊髓损伤后 6 h发现GFAP的表达增加.打击伤组仅在伤点和近伤段变化,而火器贯通伤组波及中伤段及远伤段.结论 和脊髓打击伤相似,脊髓火器伤后神经细胞的破坏与修复并存,有一定的时空性,且与损伤程度有关.受伤后6 h发现神经胶质细胞的表达增加,但脊髓火器伤的波及范围较打击伤更为广泛.
Objective To creat porcine models of gunshot SCI in thoracolumbar spine and complete paraplegia of contusion model of SCI using the modified Allen＇s method, and to investigate the early expression of galial filament acid protein （GFAP）. Methods Twenty healthy porcine were randomly divided into three groups： gunshot injury group （n = 9）, the Allen method induced injury group （n = 9） and the control group （n = 2）. Specimens were obtained from injury sites, proximal sites, middle sites and distal sites at 1 h, 3 h, 6 h after injury. Histological changes of these sites were observed with immuno-dying of GFAP. Results The level of expression of GFAP did not increase until at the point of 6 h after injury. The changes of GFAP could only be observed in injury site and the proximal site in injury group, while those changes could also be found in middle and distal site in gunshot group. Conclusions Destruction and reconstruction coexisted in the same neuron cell that happened after both kinds of injuries. This kind of activity has temporal and spatial characteristic in relation with the severity of injury. At 6th hour after injury the number of glial cells increased in gunshot models, however, the injury area of gunshot SCI would be more extensive than that of the Allen＇s method induced injury.
出处
《临床骨科杂志》
2006年第3期267-270,共4页
Journal of Clinical Orthopaedics
关键词
脊髓损伤
创伤
枪击
胶质纤维酸性蛋白
spinal cord injuries
wounds, gunshot
galial filament acid protein
