摘要
目的探讨肿瘤抗原特异性细胞毒性T淋巴细胞(CTL)对裸鼠人肝癌转移模型LCI-D20的治疗作用。方法从健康人外周血单个核细胞中诱导树突状细胞,用重组人粒细胞-巨噬细胞集落刺激因子和白细胞介素-4刺激活化,经人肝癌细胞株MHCC97-H肿瘤抗原致敏后,诱导肿瘤抗原特异性CTL,经腹腔注射,以自然杀伤样T淋巴细胞(CIK)和磷酸盐缓冲液为对照,研究其对LCI-D20肝癌治疗和预防转移作用。结果肿瘤抗原特异性CTL组、CIK 组和对照组肝癌肿块重量、血清甲胎蛋白含量、肝癌肝内转移率和存活期依次为(1．11±0．63)g、(1．12±0．36)g 和(2．68±0．53)g;(52．1±9．7)μg／L、(48．6±5．2)μg／L和(82．2±7．2)μg/L;16．7％、16．7％和58．3％; (79．0±5．0)d、(73．3±7．0)d和(52．3±5．2)d。对照组与前两组比较差异均有统计学意义(P值均<0．01)。结论肿瘤抗原特异性CTL可以预防LCI-D20模型肝癌发生转移,延长动物存活时间。
Objective To investigate the cure effect of tumor antigen specific CTL on a model of human hepatocellular carcinoma in nude mice LCI-D20. Method Dendritic cells （DCs） were induced from peripheral blood mononuclear cells of healthy people in vitro by using recombinant human granulocyte-macrophage colony stimulating factor （rhGM-CSF） and interleukin- 4 （rhIL-4） and were pulsed with tumor antigen from hepatocellular carcinoma cell line MHCC97H. Then tumor antigen specific cytotoxic T lymphocytes （CTLs） were induced. By intrapefitoneal injection of tumour antigen specific CTLs into the LCI-D20, the preventive and therapeutic effects of these CTLs to HCC in the LCI-D20 model were assessed. Cytokine-induced killer （CIK） cells and phosphate buffer solution were used as controls at the same time. Results The weights of tumors in the tumor antigen specific CTL group, in the CIK cell group and in the blank group were （ 1.11 ± 0.63）, （1.12 ± 0.36） and （2.68 ± 0.53） grams respectively （t = 5.18, t = 6.06, P 〈 0.01）. The amount of blood alpha fetal protein in the tumor antigen specific CTL and CIK goups were （52.1 ± 9.7） μg/L and （48.6 ± 5.2） μg/L, and was （82.2 ± 7.2） μg/L in the blank group （t = 17.26, t = 22.07, P 〈 0.01 respectively）. The metastasis rates in livers were 16.7%, 16.7% and 58.3% in the tumor antigen specific CTL, CIK cell and blank control groups respectively （ x^2 = 4.44, P 〈 0.01）. The survival time of the mice in the tumor antigen specific CTL group was （79.0 ± 5.02） days, （73.3 ± 7.0） days in the CIK group, and （52.3 ± 5.2） days in the blank group （t = 14.56, t = 17.54, P 〈 0.01）. Conclusion Tumor antigen specific CTLs may prevent metastasis in the LCI-D20 model and prolong the survival time.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2006年第6期431-434,共4页
Chinese Journal of Hepatology
关键词
树突细胞
T淋巴细胞
细胞毒性
抗原
肿瘤
癌
肝细胞
Dendritic cells
T-lymphocytes, cytotoxic
Antigens, neoplasm
Carcinoma, hepatocellular
