摘要
采用高效液相色谱法测定了功能性红曲米粉中的青霉素G钠,选用Aichrom Bond-AQ C18（250 mm×4.6 mm i.d.,5μm）色谱柱,V（甲醇）：V（水）=30：70为流动相,检测波长为215 nm,流速1 mL/min,结果青霉素G钠在1.0～500 mg/L范围内具有良好的线性关系,r=0.9999;回收率为99.0%～107.5%;检出限为0.2 ng.
A method for the determination of sodium penicillin G in functional red yeast rice by HPLC was developed. Chromatographic separation was carried out on an AichromBond-AQ C18 column （4.6 × 250 mm, 5 μm i. d. ） under isocratic conditions with methanol and water （30：70） as the mobile phase at a flow-rate of 1 mL/min. The UV detection wavelength was set at 215 nm. Then the experimental results showed that the linear range for sodium penicillin G was 1.0- 500 mg/L, r = 0.9999; Experimental recovery and limit of detection were 99.0% - 107.5% and 0.002 ng, respectively.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第6期78-80,共3页
Chinese Journal of Analysis Laboratory
