摘要
In order to implement industrial production of selected functional bacteria for bioremediation of shrimp culture environment, the fermentation conditions of the three functional bacteria, which have high ability in degrading organic pollutants at the bottom of shrimp ponds, were studied. The results showed that the favorable fermentation medium （per L） was 25 g of peptone, 5 g of yeast extract, and 0.2 g of ferric phosphate, and the initial pH value of the medium was 8.0. The optimum fermentation time was 20 h. The optimum stirring way was stirring one hour after one hour. Iso-electric point sedimentation collecting method was the most efficient and economic method to collect the bacteria cells after fermentation, with the optimum sediment pH of 3.67, 4.02, and 3.40 for the strains Lt7222, Gy7018 and Lt7511,respectively. It was also indicated that the survival and reproducing ability of the bacterial cells were not affected by the sedimentation process.
为了实现所筛选的对虾养殖环境生物修复作用菌的产业化生产,本文选择三株具有高效降解有机污染物能力的菌株为代表,对作用菌的发酵生产和菌体收集工艺进行了优化。结果表明,作用菌的最适发酵培养基配方(g/L)为蛋白胨 25,酵母粉 5,磷酸铁 0.2,灭菌前培养基的 pH 可先调为 8.0,搅拌方式为搅拌1h 间隔 1h, 出罐时间以 20h 为宜。菌体收集以等电点沉降法效率最高、成本最低,其中菌株 Lt7222 的最适沉降 pH 为 3.67、Gy7018 为 4.02、Lt7511 为 3.40。菌体回收率可达 90 %,回收后菌体的存活能力几乎不受影响。
