摘要
为了研究部分D表型的分子机理,用间接抗人球蛋白方法(IAT)筛选弱表达的D变异体,PCR-SSP(poly-merasechainreaction-sequencesepecificprimer)方法扩增RHD基因特异的外显子及其侧翼序列,用PCR产物直接序列分析测定核苷酸的变异。结果表明:从22例弱D中检测到10例部分D表型,其中DVa(Kou.)、DVa(Hus.)和DVa-like(YH.)各1例,DVItypeⅢ表型7例。结论:10例部分D表型的分子机理得到明确,其中DVa(Kou.)、DVa-like(YH.)表型为国内首次报道。
To investigate the molecular basis of partial D phenotypes in Chinese, D variants with weak D expression was screened by using indirect anti-human globulin test （IAT） method, the polymerase chain reaction-sequenc specific primer （PCR-SSP） method was employed to amplify RHD specific exons and their flanking regions. The amplification products were sequenced directly to determine the molecular basis of D variants. The results showed that ten cases of partial D phenotypes, including one case ofD Va（Kou. ）, one case of D Va（Hus.）, one case ofD Va-like （YH.）, and seven cases of D Ⅵ type Ⅲ, were detected from 22 cases of weak D phenotype respectively. All ten cases of partial D phenotypes had one RHD allele deleted. In conclusion, the molecular basis of ten cases of partial D phenotype was confirmed, including D Va（ Kou. ）and D Va-like （YH.）phenotypes reported firstly in Chinese population.
出处
《中国实验血液学杂志》
CAS
CSCD
2006年第3期587-591,共5页
Journal of Experimental Hematology
基金
浙江省自然科学基金项目(M303194)
