期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

干扰素-γ对人肺癌A549细胞增殖和P21分子的影响 被引量：1

Effect of IFN-γ on A549 cell proliferation and expression of P21
暂未订购
导出
摘要 目的:探讨干扰素-γ(IFN-γ)对人肺癌A549细胞增殖和P21分子的影响。方法:培养人肺癌A549细胞,与IFN-γ共孵育,通过MTT比色法观察IFN-γ对人肺癌A549细胞增殖的影响,同时通过免疫细胞化学法,分析人肺癌A549细胞中P21蛋白表达的变化。结果:与对照组相比,IFN-γ组能够明显抑制细胞增殖且呈剂量依赖性,同时免疫组化结果显示,IFN-γ可以上调P21蛋白的表达。结论:IFN-γ可以增加P21蛋白的表达,抑制人肺癌A549细胞增殖,从而可能起到抗肿瘤的疗效。 AIM： To observe the effect of IFN-γ on the A549 cell proliferation and the expression of P21 protein. METHODS： A549 cells were cultured and incubated with IFN-γ. MTT colorimetric method was used to observe the effect of IFN-γ on the A549 cell proliferation, and the change of P21 protein expression was observed by the immunocytochemistry. RESULTS： Compared with those of control group, the results of MTT showed that IFN-γ could reduce the A549 cell proliferation. From the experiment of immunocytochemistry,, the expression of P21 protein increased significantly after treatment with IFN-γ. CONCLUSION： IFN-γ can induce the expression of P21 protein and inhibit the A549 cell proliferation and maybe provide the new idea for the therapy of lung cancer.
作者 夏晖 于长海 俞建琦 李捷 张宝石 李英杰
机构地区 中国人民解放军总医院第一附属医院胸外科
出处 《中国临床药理学与治疗学》 CAS CSCD 2006年第4期424-426,共3页 Chinese Journal of Clinical Pharmacology and Therapeutics
关键词 干扰素-Γ A549细胞 肺癌 免疫治疗 IFN-γ A549 cells apoptosis lung cancer immunotherapy
分类号 R963 [医药卫生—微生物与生化药学]
  • 相关文献

参考文献6

  • 1Curnis F,Gasparri A,Sacchi A,Cattaneo A,Magni F,Corti A.Targeted delivery of IFN gamma to tumor vessels uncouples antitumor from counterregulatory mechanisms[J].Cancer Res,2005;65:2906-13
  • 2Zhang C,Zhang J,Sun R,Feng J,Wei H,Tian Z.Opposing effect of IFN gamma and IFN alpha on expression of NKG2 receptors:negative regulation of IFN gamma on NK cells[J].Int Immunopharmacol,2005;5:1057-67
  • 3Marini M,Bamias G,Rivera-Nieves J,Cominelli F.TNF-alpha neutralization ameliorates the severity of murine Crohn's-like ileitis by abrogation of intestinal epithelial cell apoptosis[J].Proc Natl Acad Sci U S A,2003 ;100:8366-71
  • 4李哲,袁守军,聂丽平,田增月,徐兰平,韩昌明.青蒿琥酯诱导肿瘤细胞凋亡与抑制存活蛋白表达有关[J].中国临床药理学与治疗学,2004,9(6):607-611. 被引量：41
  • 5Sano H,Kawahito Y,Wilder RL,Hashiramoto A,Mukai S,Asai K,et al.Expression of cyclooxygenase-1 and -2 in human colorectal cancer[[J].Cancer Res,1995;55:3785-9
  • 6Williams CS,Luongo C,Radhika A,Zhang T,Lamps LW,Nanney LB,et al.Elevated cyclooxygenase-2 levels in Min mouse adenomas[J].Gastroenterology,1996;111:1134-40

二级参考文献12

  • 1颜子颖 王海林.精编分子生物学实验指南[M].北京:科学出版社,1998.17-287.
  • 2[1]Woerdenbag HJ, Moskal TA, Pras N, Malingre TM, el-Feraly FS, Kampinga HH, et al. Cytotoxicity of arte misinin-related endoperoxides to Ehrlich ascites tumor cells [J]. J Nat Prod,1993; 56(6): 849- 56
  • 3[3]Li Y, Shan F, Wu JM, Wu GS, Ding J, Xiao D, et al. Novel antitumor artemisinin derivatives targeting G1 phase of the cell cycle[J]. Bioorg Med Chem Lett, 2001;11(1):5- 8
  • 4[7]Singh NP, Lai H. Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells[J]. Life Sciences, 2001;70(1):49- 56
  • 5[8]Moore JC, Lai H, Li JR, Ren RL, McDougall JA, Singh NP,et al. Oral administration of dihydroartemisinin and ferrous sulfate retarded implanted fibrosarcoma growth in the rat[J]. Cancer Lett, 1995;98(1):83- 7
  • 6[9]Lai H, Singh NP. Selective cancer cell cytotoxicity from exposure to dihydroartemisinin and holotransferrin [J]. Cancer Lett,1995;91(1) :41 - 6
  • 7[10]Olie RA, Simoes-Wust AP, Baunann B, Leech SH, Fabbro D, Stahel RA, et al. A novel antisense oligonucleotide targeting surviving expression induces apoptosis and sensitizes lung cancer cells to chemotherapy[J]. Cancer Res, 2000; 60 (11):2805 - 9
  • 8[11]Shin S, Sung B J, Cho YS, Kim H J, Ha NC, Hwang JI, et al. An anti-apoptotic protein human survivin is a direct inhibitor of Caspase-3 and-7[J]. Biochenistry, 2001 ;40(4): 1117 - 23
  • 9[12]Kato J, Kuwabara Y, Mitani M, Shinoda N, Sato A, Toyama T, et al. Expression of surviving in esophageal cancer: correlation with the prognosis and response to chemotherapy [J]. Int J cancer, 2001 ;95(2) :92 - 5
  • 10张星,杨小平,潘启超.青蒿酯钠抗人肝癌(BEL-7402)与诱导凋亡[J].中草药,1998,29(7):467-469. 被引量：39

共引文献40

同被引文献12

  • 1Angioli R,Sevin BU,Perras JD,et al.In vitro potentiation of radiation cytotoxicity by recombinant interferons in cervical cancer cell lines.Cancer,1993,71:3717-3725.
  • 2Mertens R, Granzen B, Lassay L, et al. Nasopharyngeal carcinoma in childhood and adolescence:concept and preliminary results of the cooperative GPOH study NPC-91.Gesellschaft für P(a)diatrische Onkologie und H(a)matologie.Cancer,1997,80:951-959.
  • 3Dawson DE,Gapany M,Burgess RC,et al.Tumor necrosis factor and chemotherapeutic agents. Potentiation of cytotoxicity with interferon gamma.Arch Otolaryngol Head Neck Surg,1992,118:1168-1171.
  • 4McDonald S,Chang AY,Rubin P,et al.Combined Betaseron R (recombinant human interferon beta) and radiation for inoperable non-small cell lung cancer.Int J Radiat Oncol Biol Phys,1993,27:613-619.
  • 5Byhardt RW,Vaickus L,Witt PL,et al.Recombinant human interferon-beta (rHuIFN-beta) and radiation therapy for inoperable non-small cell lung cancer.J Interferon Cytokine Res,1996,16:891-902.
  • 6Schmidberger H,Rave-Fr(a)nk M,Lehmann J,et al.The combined effect of interferon beta and radiation on five human tumor cell lines and embryonal lung fibroblasts.Int J Radiat Oncol Biol Phys,1999,43:405-412.
  • 7Mochimatsu I,Tsukuda M,Furukawa S,et al.The sensitivity of head and neck squamous cell carcinomas to tumor necrosis factoralpha.Biotherapy,1993,6:239-244.
  • 8Morrow AN,Schmeisser H,Tsuno T,et al.A novel role for IFN-stimulated gene factor 3 Ⅱ in IFN-γ signaling and induction of antiviral activity in human cells.J Immunol,2011,186:1685-1693.
  • 9Kurdi M,Booz GW.Jak inhibition,but not Statl knockdown,blocks the synergistic effect of IFN-gamma on Fas-induced apoptosis of A549 human non-small cell lung cancer cells.J Interferon Cytokine Res,2007,27:23-31.
  • 10Partridge M,Costea DE,Huang X.The changing face of p53 in head and neck cancer.Int J Oral Maxillofac Surg,2007,36:1123-1138.

引证文献1

二级引证文献2

中国临床药理学与治疗学
2006年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部