摘要
目的:观察白藜芦醇对K562细胞的诱导凋亡效应,探讨其可能的作用机制。方法:应用噻唑蓝(MTT)比色法、光镜、电镜观察K562细胞凋亡;流式细胞术(FCM)测定K562细胞Bcl-2、P53蛋白水平的变化;比色法检测Caspase-3活性变化。结果:白藜芦醇可抑制K562细胞增殖,光镜和电镜下观察呈典型凋亡形态改变,Bcl-2蛋白表达明显下调,Caspase-3活性明显增强。结论:白藜芦醇可通过Bcl-2依赖性Caspase-3激活而诱导K562细胞凋亡。
AIM： To observe the apoptosis of K562 cells induced by resveratrol and to explore its possible mechanisms. METHODS： MTT assay, light microscopy and electron microscopy were used to examine apoptosis in K562 cells. Expression levels of Bcl-2 and P53 antigens were mesured by FCM. Colorimetric assay was employed to detect the activation of Caspase-3. RESULTS： Resveratrol could inhibit growth of K562 cells. Typical morphological changes of apoptosis were observed through light microscopy and electron microcopy. Expression of Bcl-2 significantly decreased after application of resveratrol. Caspase-3 could also be activated by resveratrol. CONCLUSION： Resveratrol induces apoptosis in K562 cells by activating Caspase-3 via a Bcl-2-dependent pathway.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2006年第4期421-423,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
白藜芦醇
白血病
凋亡
resveratml
leukemia
apoptosis
