摘要
目的:建立抗生素诱导SPF级BALB/c小鼠胃肠道白念珠菌定植模型.方法:SPF级♀Balb/c小鼠随机饮用抗生素头孢曲松水溶液5d(120h)后,单次口服灌胃107CFU(50μL)白念珠菌以诱导其定植.并运用平板计数和基于细菌16SrDNA的PCR-DGGE技术分析小鼠胃肠道的白色念珠菌定植与微生物区系变化.结果:抗生素处理5d后,减少了胃肠道99.99%以上的可培养厌氧菌和肠杆菌,与处理前相比,有极显著差异(厌氧菌:8.53±0.31Log10CFU/gvs4.18±0.90Log10CFU/g,P<0.01;肠杆菌:3.67±0.14Log10CFU/gvs0,P<0.01),而且DGGE图谱分析显示,抗生素处理后小鼠细菌微生物区系的多样性明显减少(条带数由27-32条减少到2-7条).对抗生素处理小鼠单次口服灌胃107CFU白念珠菌2d后,在小鼠胃、小肠、盲肠和大肠检测到大量的C.albicans,数量分别为4.44±0.02Log10CFU/tissue,5.05±0.19Log10CFU/tissue,5.62±0.06Log10CFU/tissue,4.95±0.14Log10CFU/tissue,并且单次口服灌胃白念珠菌1wk后,小鼠胃肠道内仍维持有4.01±0.06Log10CFU/g的白念珠菌定植.与正常Control组和Antibioticonly组相比,Antibiotic+Candida模型组小鼠肠杆菌增殖了10到100倍(3.65±0.16Log10CFU/g,3.21±0.18Log10CFU/gvs5.42±0.33Log10CFU/g,P<0.05);同时DGGE图谱分析显示,Antibiotic+Candida模型组小鼠细菌微生物区系多样性较低(条带数Antibiotic+Candida/22-24条,Control/28-34条和Antibioticonly/27-34条),各小鼠细菌微生物区系之间的相似性为63.8%-67.0%.结论:抗生素处理诱导了胃肠道微生物区系紊乱,导致了白念珠菌在SPF级Balb/c小鼠胃肠道定植成模.
AIM： To establish a specific-pathogen-free （SPF） BALB/c mouse model of Candida albicans gastrointestinal colonization following antibiotic treatment.
METHODS： Female SPF BALB/c mice were treated for 5 days （120 h） with ceftriaxone ad libitum in drinking water, followed by a single oral gavage of 107CFU （50μ/L） C. albicans. The gastrointestinal microbiota of mouse model was analyzed by plate counts and 16S rDNA-based polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） technique.
RESULTS： The culturable anaerobic and en- teric bacterial levels were reduced significantly by 99.99% at day 5 of antibiotic treatment （anaerobe： 8.53±0.31 Logl0CFU/g vs 4.18±0.90 Log10CFU/g, P 〈 0.01; enteric bacteria： 3.67± 0.14 Logl0CFU/g vs 0, P 〈 0.01）. DGGE profile clearly indicated that the bacterial diversity of the gut microbiota was obviously decreased （from 27-32 bands to 2-7 bands）. A single oral gavage of 107CFU C. albicans resulted in C. albicans deposi- tion in stomach, small intestine, cecum and large intestine of antibiotic-treated mice at day 2 post- antibiotic treatment （4.44 ±0.02 Logl0CFU/tis- sue, 5.05± 0.19 Logl0CFU/tissue, 5.62±0.06 Logl0CFU/tissue and 4.95±0.14 Logl0CFU/tissue, respectively）, and 4.01 ＋ 0.06 Logl0CFU/g C. albicans colonization in gastrointestinal tract of antibiotic-treated mice at day 7 post-antibiotic treatment. The enteric bacterial levels of the gut mcirobiota of mice model was 10- to 100-fold- higher as compared with those in the mice untreated and antibiotic-treated only at day 7 post- antibiotic treatment （3.65±0.16 Logl0CFU/g, 3.21 ±0.18 Logl0CFU/g vs 5.42 ＋ 0.33 Logl0CFU/g, P 〈 0.05）. The analysis of DGGE profile showed that the bacterial diversity of mice model was lower （antibiotic ＋ Candida/22-24 bands, Con- trol/28-34 bands and antibiotic only/27-34 bands）, and similarity values of each mice ranged from 63.8% to 67.0%.
CONCLUSION： Antibiotic-induced microbiota disruption leads to persistence of gastrointesti- nal C. albicans colonization of SPF BALB/c mice.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第12期1161-1166,共6页
World Chinese Journal of Digestology
关键词
抗生素
白念珠菌定植
微生物区系
Antibiotic
Candida aibicans colonization
Microbiota
