摘要
目的研究环氧合酶-2(cyc looxygenase-2,COX-2)在成年大鼠毛囊皮脂腺单位中的表达模式。方法用RT-PCR、免疫组织化学技术和原代干细胞培养技术观察COX-2在毛囊皮脂腺单位的表达情况。结果成年大鼠毛囊皮脂腺单位中,bu lge(隆突)区弱表达,皮脂腺高表达,而毛球部未见表达。结论COX-2在正常成年大鼠皮脂腺中呈特异性的表达,并且它的表达模式联系毛囊干细胞从bu lge到皮脂腺迁移分化通路,提示COX-2在毛囊干细胞定向分化为皮脂腺细胞中可能起一定的作用。
Objective To investigate the expression pattern of COX-2 in adult rat pilosebaceous unit. Methods The expression pattern of COX-2 was detected by RT-PCT, immunohistochemical staining and stem cell primary culture. Results COX-2 expression in pilosebaceous unit of adult rat showed strongly positive at sebaceous gland, positive in bulge region and negative in hair matrix. Conclusion COX-2 in adult rat sebaceous gland was strongly and specifically expressed. Based on the theory that the hair follicle stem cells can migrate and differentiate from bulge region to sebaceous gland, the expression patterns of COX-2 indicates that it may play an important role on orienting differentiation of hair follicle stem cells to sebocytes.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第11期1170-1172,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目(30471568)~~
