摘要
以豌豆上胚轴(切段)、芽尖、幼叶(切半)为外殖体,诱导愈伤组织,用茄病镰刀菌粗毒素作为选择压力,离体筛选抗病变异体。结果表明:以芽尖为外植体、用M_3培养基诱导愈伤组织频率较高;间接、间歇轮回筛选法能获得较多的抗性分化植株;经苗期抗病性鉴定获得了抗病变异体41株,初步建立起了离体诱导和筛选抗豌豆根腐病变异体的实验系统。
Calli were induced from the explants of pea epicotyl segments(4～5mm), tips of shoots and the first young leaves(in halves); selected under the selection pressure of Fnsarium solani crude toxin; then transferred to regeneration medium for the occurrence of differentiation and the selection of disease—resistant variants. It was shown that a higher callus—inducing frequency was obtained in the case of using explants of shoot tips and M_3 medium and the indirect intermittent recurrent method of selection could result in a larger number of resistant variants. In this experiment 41 disease—resistant variantgs were identified through a resistance test during pot growing and a system of in vitro selection was established for obtaining pea variants resistant to root—rot.
出处
《草业学报》
CSCD
1996年第2期30-37,共8页
Acta Prataculturae Sinica
基金
甘肃省教委资助项目
关键词
豌豆
根腐病
变异体
抗病性
Pea(Pisum sativum), Fusarium solani, toxin, in vitro selection, disease resistant variants
