摘要
近些年来,海藻酸钙微胶珠在生物工程领域得到了越来越广泛的应用。为了采用海藻酸钙胶珠培养动物细胞,并给细胞提供最佳的生长环境,需要确定海藻酸钙胶珠内部的扩散系数(D)及适宜直径。今以海藻酸钙胶珠为研究对象,建立了胶珠内部的扩散传质模型,并研究胶珠尺寸或其内部孔隙结构对其扩散系数的影响。通过制作不同直径的胶珠进行葡萄糖的扩散实验,得到各种直径条件下胶珠内部的扩散数据,再通过传质模型,并结合相应的扩散数据进行计算,从而得出不同直径胶珠的内部扩散系数。实验及关联结果表明:随着所制备的胶珠直径的增加,形成海藻酸钙凝胶时钙离子置换钠离子的传质阻力在增加,因此胶珠内部钙离子含量减少,致使胶珠内部的孔道直径增大,从而使其内部扩散系数增加。在选择高扩散系数的前提下,结合动物细胞培养的要求,我们认为适用于动物细胞培养的海藻酸钙胶珠的适宜直径为2mm左右。
Calcium-Alginate Microbeads （Ca-Alg MBs） has got more and more wide applications in bioengineering fields recently. In order to culture animal cells in Ca-Alg MBs and to provide the optimum growth conditions for the cells, the diffusivity （D） and the favorable diameter of Ca-Alg MBs used should be determined. A model of diffusive mass transfer in the gel bead was established for the study of the relationship between the diameter or the inner pore structure of Ca-Alg MBs and the diffusivity. Firstly, Ca-Alg MBs of different diameters were prepared through a high-voltage electrostatic droplet generator. Then, the diffusive experiments were conducted to get the diffusive data of glucose in Ca-Alg MBs with different diameters. After that, a diffusive mathematical model was set up to find the diffusivity of glucose in Ca-Alg MBs by fitting the experimental data. And then, the diffusivity in Ca-Alg MBs with different diameters was calculated. Both the experimental results and the model calculations show that the diffusivity increases with the diameter of the Ca-Alg MBs, and the mechanism of this phenomenon was discussed. On the premises of selecting the Ca-Alg MBs with higher diffusivity and combining with the requirement of the animal cell culture, it is proposed that the proper diameter of Ca-Alg MBs used for animal cell culture should be 2 mm or so.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2006年第3期345-350,共6页
Journal of Chemical Engineering of Chinese Universities
基金
国家自然科学基金两个基地国际合作项目(2002008)
辽宁省科学技术基金资助项目(20022140)。
关键词
海藻酸钙
胶珠
扩散系数
传质
数学模型
calcium-alginate
microbeads, diffusivity
mass transfer
mathematic model
