摘要
目的建立测定固体样品中过氧化氢的方法.方法利用过氧化氢酶可专一分解H2O2为氧与水,氧电极对溶解氧具有高度选择性的原理,用磷酸盐缓冲液对样品中的过氧化氢进行提取,用N2将溶液中原来溶存的O2充分置换后,加入过氧化氢酶,用溶氧仪定量检测由过氧化氢酶分解H2O2产生的溶解氧,从而间接计算出样品中H2O2的含量.结果该方法检测的线性范围为0.5～7.5μg/ml,r＞0.999,检出限为0.13μg/ml.2个浓度H2O2的平均回收率分别为90.59%～93.88%,相对标准差＜10%.结论经过对补钙产品、莲子等干果及豆腐丝、虾皮等食品的检测,方法简便、易行.
Objective A oxygen-electrode method is developed for the determination of H2O2 residues in solid food. Methods The hydrogen peroxide can be decomposed specifically into water and oxygen by catalase. The oxygen-electrode has high selectivity with the dissolved oxygen （DO）. After H2O2 in the samples is extracted by PBS and the system is deoxidized by nitrogen, the DO can be detected by oxygen meter and then the content of H2O2 in samples will be calculated according to the result. Results The linear range is from 0.13pg/ml. The recoveries are between 90.59 %-93.88 %. BSD is below 10%. Conclusion The method has been used for the detection of H2O2 in health-preservation products of calcium, dried small shrimps, bean curd et al, it proved to be a simple rapid method.
出处
《卫生研究》
CAS
CSCD
北大核心
2006年第3期313-316,共4页
Journal of Hygiene Research
