摘要
目的了解乳酸杆菌抑菌能力的相关因素,探讨白色念珠菌与乳酸杆菌的关系及其在乳酸杆菌抑菌中的作用。方法测定阴道乳酸杆菌在不同培养时间产生H2O2的能力;用牛津杯实验筛选出抑菌能力不同的菌株,分别测定在不同培养时间,培养上清液的pH变化;用混合培养法研究菌际关系。结果pH的高低与抑菌圈的大小呈负相关;不同乳酸杆菌菌株产生H2O2的能力不同;培养上清液中的H2O2含量与抑菌圈大小关系不明显;菌际关系研究中,葡萄球菌活菌计数的菌量关系为:葡萄球菌单独培养>葡萄球菌+乳酸杆菌混合培养>葡萄球菌+乳酸杆菌+白色念珠菌混合培养。结论乳酸杆菌产酸能力与其抑菌能力成正相关,H2O2产生能力与乳酸杆菌的抑菌作用关系不明显。在培养36h后,白色念珠菌有增强乳酸杆菌抑菌能力的趋势。
Objective To study the factors linked to the bactcriostatic ability of Lactobacillus in human vagina, and the relationship between the Lactobacillus and the Candida albicans. Methods The content of H2O2 in the upper culture mediums of Lactobacilli were detected at different incubation time. Selecting the strains with vary bacteriostatic ability by Oxford cup test, detecting pH of these strains cultured after different time. After co-cuhivation of the Lactobacillus, which has the strongest bactcriostatic ability, Candida albicans and Staphylococci for different incubation time, quantities of these mixed cultures were investigated. Results The dale of pH and the size of the bactcriostatic ring are negative correlation. There is not obvious relationship between the ability of producing H2O2 and the bacteriostatic ability of Lactobacillus. The study of the relationship between hacteria shows that the quantity of Staphylococci is as this： Staphylococci alone 〉 Staphylococci ＋ Lactobacillus 〉 Staphylococci ＋ Lactobacillus ＋ Candida albicans. Conclusion The bacteriostatic ability of Lactobhcillus is positivly related to its acid producing ability, and is not obvious to H2O2 content. After 36 hours cultivation time, Candida albicans can boost up the bacteriostatic ability of Lactobacillus.
出处
《卫生研究》
CAS
CSCD
北大核心
2006年第3期310-313,共4页
Journal of Hygiene Research
