摘要
建立了青杨(Populus cathayana)组织培养系统.最适合的外植体是新切下的枝条插入含有蛭石和充足水的营养钵中24 h以上,使其在弱光下迅速长出嫩枝的茎切段和茎尖.茎切段和茎尖外植体经0.1%HgCl2或5%NaClO溶液表面消毒后,接种到大量元素减半、含0.5 mg/L 6-BA和0.03 mg/L NAA的MS培养基上诱导芽,试管苗的生根采用含0.5 mg/L IBA的MS培养基.试管苗茎段的分化依外源激素条件的不同而异.
The tissue culture system of Cathay Poplar （Populus cathayana） was developed in the present paper. The most suitable explants for in vitro growth were shoot-tips and stem segments from sprouting cutting which were plugged in water or in vermiculite under weak light more than 24 h, Surface sterilization of explants was accomplished by 0. 1% HgCl2 or 5 % NaClO. The most suitable medium for differentiation of stem segment from plantlets was the modified MS （half of macro- elements） with 0. 3 mg/L 6-BA and 0. 03 mg/L NAA and MS with 0. 3 mg/L IBA for rooting in vitro. The differentiation of stem segment of plantlets depended on the concentration of exogenous hormone.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第2期103-105,共3页
Journal of Henan Normal University(Natural Science Edition)
基金
河南省自然科学基金(0511020600)
河南省植物重点学科资助
关键词
芽
青杨
组织培养
shoot
Populus cathayana
tissue culture
