摘要
自行设计组装了一套以白色发光二极管为光源的新型表面等离子体子共振传感器,并采用此传感器通过测量动力学参数测定了8种抗生素与固定在金膜上的牛血清白蛋白之间的结合率,将所得数据与常用的平衡透析法进行了比较,两者具有良好的可比性和线性相关性,说明采用此法也可测定抗生素与蛋白的结合率.该方法具有测定周期短、样品用量少、操作简便、预测效果良好以及仪器易于小型化甚至微型化等优点.
Based on surface plasmon resonance （SPR） , a novel optical chemical sensor was developed. Traditionally, wavelength modulation SPR sensing method adapted tungsten lamp as the light source with an expensive and complicated apparatus. The device presented here is a relatively simple and inexpensive one. It was designed using a white LED with a 5 V battery as the light source. The percentage bound of 8 antibiotics with BSA was studied by this setup. The results were compared with the reliable percentage bound data obtained by standard equilibrium dialysis and a relative good agreement was achieved.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第6期1028-1031,共4页
Chemical Journal of Chinese Universities
关键词
表面等离子体共振传感器
抗生素
蛋白
结合率
Surface plasomon resonance sensor
Antibiotic
Protein
Percentage bound
