摘要
研究了啤酒酵母的光散射光谱．结果表明，在波长308．0nm处，光散射强度与啤酒酵母浓度在2．0×10^4-2．0×10^6Cell／mL范围内呈线性关系，检出限（3σ）为4．94×10^2Cell／mL．将此方法成功地应用于培养液中啤酒酵母含量的快速、灵敏的测定，并对其散射光谱进行了表征．
The light scattering（LS） spectra of Saccharomyces cerevisiae was studied. The results show that the LS intensities were in proportion to the concentrations of Saccharomyces cerevisiae at peak 308.0 nm in the concentration range of 2. 0 × 10^4-2. 0 × 10^6 Cell/mL. The detection limit （3σ） was 4. 94 × 10^2 Cell/mL. The present new method was successfully applied to the rapid, sensitive determination and characterization of Saccharomyces cerevisiae biomass in suspensions.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第6期1023-1027,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号30570465
20425517)
重庆市科委自然基金项目资助
关键词
光散射分析法
散射截面
吸收截面
啤酒酵母
生物量
Light scattering assay
Scattering cross section
Absorption cross section
Saccharomyces cerevisiae
Biomass
