摘要
以石油醚对白酒中微量的高级脂肪酸进行萃取浓缩后作柱前衍生化处理,采用WatersμBondapakTMC18液相色谱柱、紫外检测器进行检测。结果表明,测得亚麻酸、肉豆蔻酸、亚油酸、油酸、棕榈酸、硬脂酸的相对标准偏差分别为0.84%,0.42%,0.42%,0.77%,0.22%,3.07%,回收率为91.4%￣105.5%,线性范围为0￣50mg/Lr,≥0.99954。该方法简单、准确、重现性好。
Higher fatty acids in soybean-flavor liquor were determined by HPLC （The fatty acids extracted by petroleum ether and the concentrates derived, and the derivants detected on the Waters μBondapak^TM C18 HPLC Col （300 min×3.9 mm i.d., 10 μm）, UV 242 nm）. The relative standard deviations of linolenic acid, myfistic acid, linoleic acid, oleic acid, palmitic acid and stearic acid were 0.84 %, 0.42 %, 0.42 %, 0.77 %, 0.22 % and 3.07 % respectively and the recovery was 91.4 %-105.5 %.The linear relationship of calibration curve was good in the range of 0 mg/L to 50 mg/L, r ≥0.99954. The method was simple and accurate with good reproducibility.
出处
《酿酒科技》
北大核心
2006年第6期89-90,共2页
Liquor-Making Science & Technology
关键词
分析检测
高效液相色谱法
豉香型白酒
高级脂肪酸
determination
high-performance liquid chromatography （HPLC）
soybean-flavor liquor
higher fatty acids
