摘要
目的:探讨不同提取方法对黄连中小檗碱含量的影响。方法:用药典法、回流法、超声法、回流超声法4种方法提取黄连,以高效液相色谱法分别测定提取物中小檗碱的含量。结果:《中国药典》提供的方法与其它提取方法所得小檗碱含量结果有显著性差异(P<0·01)。结论:回流超声法与其它方法比较,时间短、含量高,较适宜提取黄连中的小檗碱。
OBJECTIVE：To explore the effect of different extraction methods on the content of berberine hydrochloride in Rhizoma Coptis.METHODS： Berberine hydrochloride in Rhizoma Coptis was extracted respectively by China Pharmacopoeia extraction method, reflux method, ultrasound wave method and refluxing ＆ ultrasonic wave extracting method,and the content of which was determined by HPLC.RESULTS：There were significant differences in the extraction content of berberine hydrochloride between the extraction method stated in China Pharmacopoeia and the other extraction methods（ P〈0.01）. CONCLUSIONS： As compared with the other extraction methods, the refluxing ＆ ultrasonic wave extraction method has the advantages of shorter time, higher content of extract, and it is more suitable for the extraction of berberine hydrochloride from Rhizoma Coptis.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第11期859-860,共2页
China Pharmacy
关键词
提取方法
黄连
小檗碱
含量测定
高效液相色谱法
Extraction method
Rhizoma Coptis
Berberine hydrochloride
Content determination
HPLC
