摘要
目的:建立HPLC法测定珍菊降压片中氢氯噻嗪和芦丁的含量。方法:用Lbicbrospher C18柱(200 mm×4．6 mm,5μm),以0．05 M枸橼酸溶液-乙腈(82:18)为流动相,流速:1．0 ml·min-1,检测波长:270 nm。结果:氢氯噻嗪线性范围0．50- 4．03μg(r=0．999 9),平均加样回收率99．9％(RSD=0．7％)、芦丁线性范围1．99-15．94μg(r=0．999 6),平均加样回收率98．7％(RSD=0．8％)。结论:该方法简单,快速,可控制本品的质量。
Objective： To establish a HPLC method for determination the content of hydrochlorothiazide and rutin in zhenjujianya tablish. Method. The Lichrospher C 18 column （200 mm× 4.6 mm,5 μm） was used, the 0.05 mol. L^- 1 citric acid solution-acetonitrile （82：18） as mobile phase, the flow rate was 1.0 ml· min^-1, the detection wavelength was 270 nm. Result： The linear ranges of hydrochlorothiazide and rutin were 0.50-4.03μg（r = 0.999 9） and 1.99-15.94μg（ r = 0.999 6） respectively, the average recoveries were 99.9% （RSD 0.7% ） and 98.7 （RSD 0.8% ）, respectively. Conclusion： the method is simple and rapid, and it can be used for quality control of zhenjujianya tablets.
出处
《中国药师》
CAS
2006年第6期531-533,共3页
China Pharmacist
关键词
珍菊降压片
HPLC
含量测定
Zhenjujanya tablets
HPLC
Content dertermination
