摘要
奶牛隐性乳房炎的检测方法有多种,相比之下,近年来,国外报道的用检测奶样中髓过氧化物酶(myeloperoxidase,MPO)来诊断奶牛隐性乳房炎的方法更符合快速、简便、高灵敏度、高特异性的现代诊断方法要求。根据乳房炎过程中的生理生化指标的变化特点,可得出奶液中MPO是乳房炎指示物。奶样中的体细胞数(SCC)和MPO浓度的相关系数值为0.91,奶样中随着SCC的增高,MPO的水平也增高达到2 000 ng/mL,同时也可分离到大量细菌。免疫学分析MPO单克隆抗体与奶液中成分乳铁蛋白、乳过氧化物酶、牛血清白蛋白、MPO、淋巴细胞、少量体细胞的乳样之间ELISA反应没有交叉性,表明MPO分子在乳房炎中具有很高的特异性。通过对牛奶中MPO的检测来判断乳房内中性粒细胞(PMN)的活性状态,可对奶牛隐性乳房炎做出早期诊断。
This article evaluates the feasibility that through detecting MPO in milk of dairy cow to diagnosis latent mastitis of dairy cow. The change of physio-biochemical indices and the indicator MPO in the development of mastiffs are systematically discussed. For the milk samples the correlation coefficient between the somatic cell count and MPO concentrations determined using the ELISA was 0.91. In milk samples with a high SCC,MPO levels were also high, reaching levels of 2 000 ng/mL. Microorganisms were isolated in the milk samples with elevated concentrations of MPO and high SCC. According to ELISA analysis, the moncolonal antibodies specific to bovine MPO showed no cross reactivity with other neutrophil granule proteins such as lactoferrin, lactoperoxidase and serum albumin. The result showed that MPO is very specific and the quantitative analysis of the amount of MPO in mastitis milk can be used to detect intramammary latent infections in dairy cow.
出处
《动物医学进展》
CSCD
2006年第6期54-57,共4页
Progress In Veterinary Medicine
