摘要
目的:探讨人胚脑源性神经干细胞和脊髓源性神经干细胞的体外培养和分化的差异。方法:从人胚脑组织和脊髓组织中分离培养神经干细胞,分为EGF组、bFGF组、EGF±bFGF组,在连续传代过程中观察并比较神经干细胞体外培养特性的差异:用血清诱导神经干细胞分化,观察其分化状况的不同。结果:从人胚脑组织分离的细胞在bFGF 单独存在时无法形成神经球,在EGF或EGF±bFGF存在时形成大量具有连续增殖能力的神经球;从人胚脊髓组织分离的细胞在EGF单独存在时无法形成神经球,在bFGF单独存在时只形成少量神经球,在EGF±bFGF存在时形成大量具有连续增殖能力的神经球。同样在EGF±bFGF存在的情况下,脑源性于细胞的增殖速度较快。经血清诱导后,脑组织来源的干细胞分化为NSE阳性细胞数明显多于脊髓组织来源的干细胞,二者之间的差异具有显著性(P<0．05)。结论:脑源性和脊髓源性神经干细胞在生长和分化方面有明显差别:脑源性神经干细胞可在bFGF或EGF士bFGF存在的情况下长期传代,而脊髓源性神经干细胞只能在EGF±bFGF存在的情况下长期传代,脑源性干细胞的增殖能力明显高于脊髓源性干细胞;脑源性干细胞较脊髓源性干细胞更易分化为神经元。
Objective： To explore the difference of the cultivation and differentiation in vitro of neural stem cells between brain-derived and spinal cord-derived from human embryoes. Methods： Neural stem cells were isolated from brain and spinal cord of human embryos in a culture medium containing EGF, bFGF and EGF± bFGF and were induced to differentiation by serum. Their growth and differentiation characteristics were invedtigated in continuous passages. Results： In the culture of human embryoes brain, bFGF alone does not produce any neurospheres. EGF or EGF± bFGF produce a lot of neurospheres with the potential to expand. In the culture of human embryoas spinal cord. EGF alone does not produce any neurospheres, bFGF alone produces a few neurospheres. Both EGF and bFGF produce a lot of neurospheres with the potential to expand. The cell number of brain-derived neural stem cell were more than those of spinal cord-derived. Induced by serum, the number of NSE positive cells in brain-dirived neural stem cell were more than those of spinal cord-derived （P〈0.05） . Conclusion.. There were some different characteristics between brain-derived and spinal cord-derived neural stem cells. The brain-derived neural stem cells could passage in the presence of EGF or EGF±bFGF. The spinal cord-derived neural stem cells could passage in the presence of EGF± bFGF only. The proliferative activity of brain-derived neural stem cells were higher than that of spinal cord-derived. More brain-derived neural stem cells differentiate into neurons.
出处
《脑与神经疾病杂志》
2006年第3期183-186,共4页
Journal of Brain and Nervous Diseases
基金
湖北省卫生厅科研基金重点项目资助(JX2A23)
