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蛋白质分子体外定向进化研究进展 被引量：2

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作者 孙博 王捷
机构地区 广州军区广州总医院医学实验科
出处 《实用医学杂志》 CAS 2006年第11期1335-1336,共2页 The Journal of Practical Medicine
基金 广东省自然科学基金研究团队项目(编号:20023001)
关键词 蛋白质分子 定向进化 体外 天然蛋白质 多功能
分类号 Q816 [生物学—生物工程]
  • 相关文献

参考文献18

  • 1ZHANG J H,DAWES G,STEMMER W P.Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening[J].Proc Natl Acad Sci USA,1997,94(9):4504-4509.
  • 2徐卉芳,张先恩,张用梅,A.E.G.CASS.体外分子定向进化研究进展[J].生物化学与生物物理进展,2002,29(4):518-522. 被引量：28
  • 3NEYLON C.Chemical and biochemical strategies for the randomization of protein encoding DNA sequences:library construction methods for directed evolution[J].Nucleic Acids Res,2004,32(4):1448-1459.
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  • 6FORTIN P D,MACPHERSON I,NEAU D B,et al.Directed evolution of a ringcleaving dioxygenase for polychlorinated biphenyl degradation[J].J Biol Chem,2005,280(51):42307 -42314.
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  • 8SHAO Z,ZHAO H,GIVER L,et al.Random-priming in vitro recombination:an effective tool for directed evolution[J].Nucleic Acids Res,1998,26(2):681-683.
  • 9张红缨,孔祥铎,张今.蛋白质工程的新策略——酶的体外定向进化[J].科学通报,1999,44(11):1121-1127. 被引量：17
  • 10ZHAO H,GIVER L,SHAO Z,et al.Molecular evolution by staggered extension process (StEP) in vitro recombination[J].Nat Biotechol,1998,16(3):258 -261.

二级参考文献50

  • 1[1]Zhang J-H, Dawes G, Stemmer W P C. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening. Proc Natl Acad Sci USA, 1997, 94(9):4504～4509
  • 2[2]Chen K, Arnold F H. Enzyme engineering for nonaqueous solvent: random mutagenesis to enhance activity of sublisin E in polar organic media. Bio/Technology, 1991, 9:1073～1077
  • 3[3]You L, Arnold F H. Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide. Protein Eng, 1996, 9(1):77～83
  • 4[4]Stemmer W P C. Rapid evolution of a protein in vitro by DNA shuffling. Nature, 1994, 370(4):389～391
  • 5[5]Moore J C, Jin H M, Kuchner O, et al. Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences. J Mol Biol, 1997, 272(3):336～347
  • 6[6]Crameri A, Raillard S A, Bermudez E, et al. DNA shuffling of a family of genes from diverse species accelerates evolution. Nature, 1998, 391(3):288～291
  • 7[7]Zhao H, Giver L, Shao Z, et al. Molecular evolution by staggered extension process(StEP) in vitro recombination. Nat Biotechnol, 1998, 16:258～261
  • 8[8]Shao Z, Zhao H, Arnold F H. Random-priming in vitro recombination: an effective tool for directed evolution. Nucleic Acids Res, 1998, 26(2):681～683
  • 9[9]Coco W M, Levinson W E, Crist M J, et al. DNA shuffling method for generating highly recombined genes and evolved enzymes. Nat Biotechnol, 2001, 19:354～359
  • 10[10]Abecassis V, Pompon D, Truan G. High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast. Nucleic Acids Res, 2000, 28(20):e88

共引文献51

同被引文献26

  • 1Jonczyk W,Studniarski K.Enzyme dehairing of pigskins.Soc Leather Technol Chemists,1986,72:83-88.
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  • 4Wang HY,Liu DM,Liu Y,Cheng CF,Ma QY,Huang Q,Zhang YZ.Screening and mutagenesis of a novel Bacillus pumilus strain producing alkaline protease for dehairing.Lett Appl Microbiol,2007,44:1-6.
  • 5张义正,刘德明,李珉,刘彦,程昌凤,黄庆.脱毛蚩白酶、其制造方法、使用方法和生产此蛋白酶的微生物.中国,ZL00120641.9.2007.
  • 6Huang Q,Peng Y,Zhang YZ.Purification and characterization of an extracellular alkaline serine protease with dehairing function from Bacillus pumilus.Curr Microbiol,2003,46:169-173.
  • 7张义正,黄庆,李珉,潘皎,王海燕.脱毛蛋白酶和编码这种脱毛蛋白酶的多核苷酸.中国,ZL03135279.0.2008.
  • 8Pan J,Huang Q,Zhang YZ.Geue cloning and expression of an alkaline serine protease with dehairing function from Bacillus pumilus.Curr Microbiol,2004,49:165-169.
  • 9Liu CJ,Zhang YZ.Construction of a Escherichia coil-Bacillus subtilis shuttle vector pSUGV4.J Sichuan Univ Nat Sci Ed,2001,38(2):243-245.
  • 10Hiroaki I,Nojima H,Okayama H.High efficiency transformation of Escherichia coli with plasmids.Gene,1996,96:23-28.

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二级引证文献7

实用医学杂志
2006年 第11期

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