摘要
目的:探讨雌激素受体(ER)基因XbaⅠ及PvuⅡ多态性与广州地区部分汉族绝经后妇女骨密度相互关系。方法:随机筛选年龄42～75岁广州汉族妇女157例,采用双能X线吸收法测其全身、腰椎2～4、股骨颈、Ward'三角和大转子区等部位的骨密度值,并采用聚合酶链反应限制性片段长度多态性方法检测其外周血白细胞基因组ER基因型。结果:157例受试对象中,ER基因XbaⅠ及PvuⅡ基因型分布均符合Hardy-Weinberg定律。携带XXPp基因型的个体在多个部位拥有较高的骨密度值,差异有统计学意义。结论:ER基因XbaⅠ及PvuⅡ多态性与广州汉族绝经后妇女的骨密度有一定的相关性,XXPp是一种对骨量有益的基因型。
Objective To study the relationship between the polymorphism of XbaⅠ and Pvu Ⅱ genotypes in estrogen receptor （ER） in postmenopausal women and their bone mineral density （BMD）. Methods 157 aged 42 - 75 women from Chinese Han ethnic group in the Guangzhou area were recruited to the study. For each study subject, BMD of total skeleton, lumbar vertebrae （L2.4）, and femur （greater trochanter, the neck, and the Ward＇s triangle） were measured by dual-energy X-ray absorptiometry （DEXA）. The genotypes of estrogen receptor gene were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RELP）. Results The distribution frequencies of Xba Ⅰ and Pvu Ⅱ alhles were in the Hardy-Weinberg equilibrium. The subjects with XXPp genotype had significantly higher BMD at various skehtal sites compared with those without XXPp. Conclusion The data suggested that there is some association between the polymorphism of Xba Ⅰ and Pvu Ⅱ genotypes and BMD in Han ethnic postmenopausal women in Guangzhou city.
出处
《实用医学杂志》
CAS
2006年第11期1249-1250,共2页
The Journal of Practical Medicine
基金
广东省科技计划项目(编号:2001C30706
2002B31101)
关键词
骨质疏松
绝经后
受体
雌激素
基因多态性
骨密度
Osteoporosis, postmenopausal Receptors, estrogen Gene polymorphism Bone mineral density
