摘要
编码牛免疫缺陷病毒（ＢＩＶ）穿膜蛋白的一个４００ｂｐ的基因片断被克隆到ｐＡＴＨ表达载体上，此重组的融合蛋白ＴｒｐＥ－Ｅｎｖ８在大肠杆菌中得到高效表达，而且包含有与ＢＩＶ抗体特异性反应的抗原决定簇．我们用此融合蛋白免疫ｂａｌｂ／ｃ，小鼠得到与ＢＩＶ穿膜蛋白特异性反应的杂交瘤．此单克隆抗体在蛋白印迹法（ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ）分析中与重组的ＴｒＰＥ－Ｅｎｖ８有特异性反应，用免疫酶染色法可检测ＢＩＶ在体外的复制．
A 400bp gene segment encoding a partial peptide of Bovine Immunodeficiency Virus (BIV)transmembrane protein was cloned into pATH expression vector. The recombinant fusion protein trpE-Env8 expressed at a high level in E. colt and containing a major immunoreactive epitope. The fusion protein was used to immunize balb/c mice to generate hybridoma specifically against BIV transmembrane protein. The Mab was specific for the TrpE-Env8 protein in western blotting assay and detected specifically the BIV replication in vitro by immunoperoxidase assay.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
1996年第1期59-63,共5页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家教委优秀年轻教师基金
天津市自然科学基金
关键词
牛
免疫缺陷病毒
穿膜蛋白
单克隆抗体
杂交瘤
Bovine Immunodeficiency Virus (BIV)
transmembrane protein gp45
monoclonal antibody
