摘要
用体内自发突变，卡那霉素抗性筛选和体外限制酶切富集方法消除载体ｐＮＧ３５中新霉素磷酸转移酶基因内的限制酶位点ＨｉｎｄⅢ，除去ＨｉｎｄⅢ位点后的载体ＰＮＧ３５△Ｈ赋予宿主细胞的卡那霉素抗性水平远远高于原载体ｐＮＧ３５．
Author describes the method and result of removing Hind Ⅲ site in the neomycin phosphotransferase (NPT--I) gene through spontaneous mutation in vivo, kanamycin --selection and restriction enzyme digestion--enriching mutant in vitro. A plasmid pNG35△H without Hind Ⅲ site in NPT--I gene was obtained. This plasmid confers host cells much higher kanamycin--resistance level than the plasmid pNG35.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
1996年第2期197-200,共4页
Journal of Sichuan University(Natural Science Edition)
