摘要
目的：探求ＬＡＫ细胞对白血病疗效的监测手段。方法：用流式细胞仪测定５例成人脾淋巴因子激活的杀伤细胞（ＬＡＫ细胞）和２１例正常人外周血淋巴细胞的ＤＮＡ指数（ＤＩ）及增殖周期。结果：人脾ＬＡＫ细胞的增殖活力明显高于未激活的脾淋巴细胞和外周血淋巴细胞。培养（１～１４ｄ）期间人脾ＬＡＫ细胞的增殖活力高，并且稳定。在人脾ＬＡＫ细胞中，未找出非整倍体细胞，ＤＩ指数在正常范围。结论：流式细胞仪可用于判断人脾ＬＡＫ细胞对白血病的疗效。
This research was aimed to find out a measure for observing the effect of lymphokine-activated killer (LAK ) cells on leukemia. METHODS: The DNA index (DI) and preliferative cycle of splenic LAK cells from 5 adults and peripheral lymphocytes from 21 normal subjects were estimated with flow cytometry (FCM ). RESULTS: The proliferative activity of splenic LAK cells was significantly higher than that of splenic cells and peripheral lymphocytes. It was highly active and stable during culture for a period of 1-14 d. Non-ploid cells were not found in the splenic LAK cells and the DI remained in normal range. CONCLUSIONS:FCM may be used to monitor the effect of allogenic LAK cells on leukemia.
出处
《南京铁道医学院学报》
1996年第1期4-6,共3页
Journal of Nanjing Railway Medical College
基金
江苏省科委资助
关键词
杀伤细胞
流式细胞仪
白血病
lymphokine-activated killer cells
flow cytometry
leukemia
