摘要
利用共焦显微拉曼方法,测定了乳腺组织切片的Mapping拉曼谱,光谱采样体积达到μm尺度。通过对这些共焦拉曼谱与商业提纯肌动蛋白、胶原等生化物质做出的谱的分析比较,分离出了来自细胞质、细胞间质、脂肪以及乳腺沉积物这些组织形态基元的拉曼谱;用K阶簇分析方法,还获得了细胞核基元谱;基元谱与提纯化学物质谱的相关分析印证了组织形态基元谱的来源。还分析总结了这些组织形态基元谱的特征。这些工作为深入理解组织形态与其拉曼谱的关系以及乳腺组织形态拉曼模型的建立奠定了基础。
Breast tissue sections were examined by means of confocal Raman spectroscopy with an excitation wavelength of 633 nm. Acquired using a microscopic mapping approach with the sample volume of -2 μm^3 , these spectra were compared with the ones of the commercially available actin, DNA, collagen （type Ⅰ ）, triolein etc. Some spectra were distinguished and identified and characterize the morphological elements like cell cytoplasm, extracellular matrix etc. The cell nucleus spectrum was also obtained by K-means cluster analysis. The correlation analysis showed that the spectrum from a morphological element is highly correlated with that from the corresponding purified chemical. The spectroscopic characterization of these morphological elements was then investigated. This study is helpful to understanding the chemical/morphological basis of the Raman spectrum and designing the Raman microspectroscopic model of human breast tissue.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2006年第5期869-873,共5页
Spectroscopy and Spectral Analysis
基金
国家教育部"振兴计划"资助项目
