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糖尿病大鼠肾脏细胞外基质金属蛋白酶诱导因子和基质金属蛋白酶2的表达 被引量：2

Expression of EMMPRIN and MMP-2 in diabetic rat's kidneys
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摘要 目的:研究糖尿病大鼠肾脏组织中细胞外基质金属蛋白酶诱导因子(EMMPR IN)和基质金属蛋白酶2(MMP-2)的表达,探讨糖尿病肾病的发病机制。方法:将SD大鼠随机分成正常对照组(NC组)和糖尿病模型组(DM组),DM组腹腔注射链脲佐菌素(55 mg.kg-1)诱导糖尿病大鼠模型,于第4、第8周分批处死大鼠,用免疫组化检测EMMPR IN、MMP-2表达,图像分析仪分析。结果:正常大鼠EMMPR IN、MMP-2主要表达在肾小球系膜细胞、内皮细胞、足细胞、球囊壁层上皮细胞、肾小管上皮细胞,造模后4、8周时DM组大鼠EMMPR IN、MMP-2表达较正常对照组明显下降。结论:糖尿病时肾小球EMMPR IN和MMP-2表达水平的降低,可能是引起细胞外基质积聚的原因之一。 Objective To investigate the expression of extracellular matrix metalloproteinase inducer （EMMPRIN） and matrix metalloproteinase-2 （MMP-2） in diabetic rat＇ s kidneys. Methods Rat diabetic model was induced by streptozotocin （55 mg · kg ^-1 ） , and kidneys were examined pathologically and the expression of EMMPRIN and MMP-2 was studied by immunohistochemistry. The intensity of EMMPRIN and MMP-2 was analyzed by imaging quantitative a- nalysis technique. Results EMMPRIN and MMP-2 were mainly expressed in the mesangial cells, endothelial cells, podocytes, parietal epithelial cells of Bowman＇ s capsule, and tubular cells. The expression of EMMPRIN and MMP-2 was significantly weakened in the glomeruli of diabetic rats than that of the control animals （ P 〈 0.05 ）. Conclusion Expression of EMMPRIN and MMP-2 in glomeruli is markedly decreased in diabetic rats, which is involved in the development of diabetic nephropathy .
作者 倪海锋 邓红 樊翔 李懿萍 李海浪 陈平圣
机构地区 东南大学基础医学院病理学与病理生理学系 浙江大学医学院环境基因组中心病理学与病理生理学系 东南大学附属中大医院儿科
出处 《东南大学学报（医学版）》 CAS 2006年第3期159-163,共5页 Journal of Southeast University(Medical Science Edition)
基金 国家自然科学基金资助项目(30370658)
关键词 糖尿病 细胞外基质金属蛋白酶诱导因子 基质金属蛋白酶2 表达 大鼠 diabetic mellitus extracellular matrix metalloproteinase inducer matrix metalloproteinase- 2 expression rats
分类号 R587.2 [医药卫生—内分泌] R-33 [医药卫生]
  • 相关文献

参考文献11

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二级参考文献57

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共引文献23

同被引文献20

  • 1邓红,李海浪,王凯,曾玉.茶多酚对高糖时内皮细胞产生活性氧和TGF-β_1的影响[J].江苏医药,2005,31(4):252-254. 被引量：7
  • 2曾玉,邓红,王筠,苏宁,刘东风.高糖对人脐静脉内皮细胞GSH-PX活力、NOS及ICAM-1表达的影响[J].东南大学学报（医学版）,2005,24(3):151-155. 被引量：10
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二级引证文献2

东南大学学报（医学版）
2006年 第3期

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