摘要
目的:研究整合素αvβ3在乳腺癌中的表达及抗整合素αvβ3抗体LM 609在乳腺癌骨髓微转移中的作用。方法:免疫组织化学SABC法检测69例女性乳腺癌患者肿瘤组织整合素αvβ3表达及RT-PCR法检测乳腺癌骨髓微转移状况。流式细胞仪检测人乳腺癌细胞株MCF7、MDA-MB-231、MDA-MB-435s整合素αvβ3的表达。建立BALB/c裸鼠乳腺癌骨髓微转移模型。实时荧光定量RT-PCR检测抗整合素αvβ3抗体LM609对裸鼠乳腺癌骨髓微转移模型的影响。结果:乳腺癌标本免疫组化结果,整合素αvβ3阳性患者30例,占43.5%;阴性患者39例,占56.5%。乳腺癌骨髓微转移RT-PCR检测阳性患者22例,占31.9%,阴性患者占68.1%。卡方检验,2χ=9.564,P<0.05。两者有显著相关关系。MCF-7细胞膜上整合素αvβ3受体为阴性,MDA-MB-231、MDA-MB-435s细胞膜上整合素αvβ3受体阳性。在由整合素αvβ3受体阳性细胞制作的裸鼠乳腺癌骨髓微转移模型中,经LM609处理后,可显著减少乳腺癌骨髓微转移的肿瘤细胞(P<0.05)。结论:整合素αvβ3在乳腺癌中高表达与骨髓微转移有显著相关关系,用抗整合素αvβ3抗体可抑制乳腺癌的骨髓微转移。
Objective： To investigate the expression of integrin αvβ3 receptor in breast cancer and to study the relationship between integrin αvβ3 and bone marrow micrometastasis. Methods： Expression of integrin αvβ3 in breast tumor was detected by SABC immunocytochemical assay. Bone marrow micrometastasis in breast cancer was tested by RT-PCR method. Flow cytometry was used to cxamirle the expression of integrin αvβ3 receptor in breast cancer cell lines MCF7, MDA-MB-231 and MDA-MB-435s. The role of LM609 in bone marrow micrometastasis in athymic mice was detected by quantitative real-time RT-PCR. The statistical analyses were performed using chi-square test and t test. Results：Overall,43.5% of tumors were integrin αvβ3 positive and 31.9% were present with bone marrow micrometastasis. The positivity of integrin αvβ3 was significantly connected with bone marrow micrometastasis. Integrin αvβ3 expression in cellular membrane was high in MDA-MB-231 and MDA-MB-435s cell lines and low in MCF-7 cell line. After the treatment with LM609, the tumor cells of bone marrow micrometastasis were fewer than those in control group in high integrin αvβ3 expression tumor cells in nude mice model （P 〈 0.05）. Conclusion：Applying the antibody of integrin αvβ3 receptor is a new strategy to inhibit bone marrow micrometastasis in breast cancer.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第6期417-420,430,F0002,共6页
Journal of Nanjing Medical University(Natural Sciences)
关键词
整合素ΑVΒ3
乳腺肿瘤
骨髓
微转移
integrinαvβ3
breast neoplasm
bone marrow
micrometastasis
