摘要
目的:使用VEGF shRNA真核表达载体转染肝癌细胞系SMMC-7721,观察对其增殖影响。方法:使用VEGF shRNA真核表达载体转染肝癌细胞系SMMC-7721,ELISA法检测转染前后肝癌细胞系SMMC-7721的VEGF蛋白表达量,检测转染前后肝癌细胞系SMMC-7721的增殖及细胞的克隆形成能力的变化。结果:VEGF shRNA真核表达载体大部分阻断肝癌细胞系SMMC-7721的VEGF蛋白表达,转染后细胞周期中,G1期细胞增多,S期细胞减少,细胞增殖能力降低;MTT法观察细胞生长速度变慢,单个细胞的克隆形成能力明显下降。结论:VEGF shRNA真核表达载体可明显抑制SMMC-7721细胞增殖能力,为将VEGF shRNA真核表达载体应用于肝癌的基因治疗提供依据。
Objective：To investigate the effects of human hepatocellular carcinoma cell line SMMC-7721 transfected with eukaryotic expression vector carrying VEGF shRNA. Methods：The vector was transfected into SMMC-7721 and the expression of VEGF of SMMC-7721 before and after transfection was detected by ELISA. The biological characteristics of SMMC-7721 before and after the transfection were observed. Results：The expression of VEGF of SMMC-7721 was blocked by VEGF shRNA,and the G1 phase cells were increased and the S phase ceils were decreased in cell cycle,and the clone formation ability of single cell was decreased after transfection.Conclusion：VEGF shRNA eukaryotic expression vector might have potential role in the gene therapy for hepatocellular carcinoma.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第6期403-406,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省教育厅高校自然科学基金资助项目(03KJB320087)
关键词
血管内皮生长因子
RNA干扰
肝肿瘤
vascular endothelial growth factor
RNAi
hepatocellular carcinoma
