摘要
目的:观察复方中药降尿酸合剂干预高尿酸血症大鼠后,大鼠血尿酸水平变化,并分析该变化是否具有剂量依赖性。方法:实验于2003-07/2004-05在青岛大学医学院附属医院动物实验室完成。①选用健康雄性Wistar大鼠48只。按随机抽签法分为6组:对照组,高尿酸血症组,降尿酸合剂低、中、高剂量组,苯溴马隆组。②对照组和高尿酸血症组给予羧甲基纤维素钠乳剂(蒸馏水配制)每次1mL/100g,2次/d;降尿酸合剂低、中、高剂量组分别给予由青岛大学医学院附属医院药剂科制备提供的复方中药降尿酸合剂。实验过程中所用中药萆榭、土茯苓、红花、薏苡仁、怀牛膝等均一次性采购,制剂科制剂人员负责药品质量控制及制剂工艺:各生药混匀,水煎两遍,取两次溶液混匀分装,250mL/瓶,高温消毒后封瓶,置冰箱中保存备用。按国家中药管理局的相关规定,以人鼠单位体质量用药量直接换算后剂量的5,10,20倍作为低、中、高剂量(5.5,11.0,23.0mL/kg,2次/d);苯溴马隆组给予苯溴马隆乳悬液5mL/kg,2次/d,连续灌胃4d。于给药后第1天给予对照组羧甲基纤维素钠乳剂(生理盐水配制)10mL/kg,其余各组给予25g/L氧嗪酸钾10mL/kg,1h后灌胃给药同前,灌胃给药后1h大鼠断尾取血,应用Sysmexchemix180生化分析仪采用尿酸酶-EHSPT法测定血尿酸。③计量资料差异比较采用方差分析,组间两两比较采用Q检验。结果:由于取血样失败,对照组,降尿酸合剂低、高剂量组,苯溴马隆组各脱失1只,最终进入结果分析:高尿酸血症组和降尿酸合剂中剂量组各8只,对照组和降尿酸合剂低、高剂量组及苯溴马隆组各7只。血尿酸水平:高尿酸血症组,降尿酸合剂低、中、高剂量组,苯溴马隆组明显高于对照组(P<0.01);降尿酸合剂高剂量组和苯溴马隆组明显低于高尿酸血症组(P<0.05,0.01),降尿酸合剂低、中剂量组虽低于高尿酸血症组,但差异不明显(P>0.05);降尿酸合剂低、中、高剂量组均高于苯溴马隆组,但差异不明显(P>0.05)。结论:降尿酸合剂可以降低高尿酸血症大鼠的血尿酸水平并呈剂量依赖性。
AIM： To observe the interventional effect of compound Chinese herb jiang niaosuan mixture on changes of hematuria of rats with hypouricemia and analyze whether the changes having dosage-dependence or not.
METHODS： The experiment was completed at Aninal Laboratory of Affliated Hospital of Medical College of Qingdao University from July 2003 to May 2005. ① Totally 48 healthy male Wistar rats were divided into 6 groups according to randomized picking method： control group, hyperuricemic group, low, middle and high dosage of jiang niaosuan mixture groups, and bensbromarone group. ② Rats in control group and hyperuricemic group were treated with 1 mL/100 g carboxymethylcellulose sodium （dispensed with distilled water） for twice a day. Rats in three dosage groups were treated with jiang niaosuan mixture provided by Agent Department of Affiliated Hospital of Medical College of Qingdao University. During study, Chinese herbs such as bixie, tufuling, honghua, yiyiren and huainiuxi were purchased one-off, and quality control and agent technique were also regulated. Raw materials were mixed and fried twice, then the liquid was mixed together and filled with 250 mL/bottle. The bottle was sealed after disinfection at hyperthemia for preparation in refrigerator： The dosages as 5, 10 and 20 times as standard body mass of human and rats were divided into low, middle and high levels （5.5, 11.0 and 23.0 mL/kg, twice a day） according to relevant law of National Administration of Traditional Chinese Medicine. Rats in bensbromarone group were perfused with 5 mL/kg bensbromarone suspension twice a day for 4 days. On the 1^st day of administration, rats in control group were treated with 10 mL/kg carboxymethylcellulose sodium （dispensed with saline）, and rats in other groups were treated with 10 mL/kg oteraeil potassium （25 g/L）. One hour later, rats were perfused with the same material mentioned above, and tail of rats was cut off to obtain the blood. Hematuria acid was determined with uricase-EHSPT method by Sysmex chemix 180 biochemical analyzer. ③ Measurement data were compared with analysis of variance, and differences between two groups were compared with Q test.
RESULTS： Four rats were lost in control group, low and high dosage groups and bensbromarone group respectively because of failing selection of blood sample. Therefore, 8 rats in hyperuricemic group and middle dosage group respectively and 7 in control group, low and high dosage groups respectively entered the final analysis. Level of hematuria acid was higher in hyperuricemic group, three dosage groups and bensbromarone group than that in control group （P 〈 0.01）; level in high dosage group and bensbremarone group was lower than that in hyperuricemic group （P 〈 0.05, 0.01）; level in low and middle dosage groups was lower than that in hyperuricemic group, but there was not significantly different （P 〉 0.05）; level in three dosage groups was higher than that in bensbremarene group, but there was not significantly different （P 〉 0.05）.
CONCLUSION： Jiang niaosuan mixture can decrease levels of hematuria acid of rats with hypouricemia and has dosage-dependence.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第19期105-107,共3页
Chinese Journal of Clinical Rehabilitation
