摘要
目的观察永生化胎肝细胞及胚胎肝细胞经脾脏移植对D-氨基半乳糖(D-gal)诱导的急性肝衰竭大鼠的治疗作用.方法采用D-gal诱导大鼠急性肝衰竭,分3组进行治疗实验,Ⅰ组经脾脏移植永生化胎肝细胞2×107个;Ⅱ组移植原代胚胎肝细胞2×107个;Ⅲ组脾脏注射生理盐水1mL,各组大鼠同时肌肉注射环孢霉素A(CsA)10mg/kg.观察大鼠的存活率、肝脏功能、肝脏病理变化情况.结果Ⅰ组及Ⅱ组大鼠存活率均显著高于Ⅲ组大鼠(72.2%vs22.2%,61.1%vs22.2%,P<0.01),肝脏功能及肝脏病理均有改善,Ⅰ组与Ⅱ组之间大鼠存活率无明显差异(72.2%vs61.1%,P>0.05).结论经脾脏移植胚胎肝细胞可明显改善D-gal诱导的肝衰竭大鼠的存活率,改善肝功能及肝脏病理,该永生化胎肝细胞可以成为肝细胞移植研究中的理想细胞材料.
AIM： To study the roles of immortalized and primary human fetal hepatocyte intrasplenical transplantations in treating the rats with acute liver failure （ALF） induced by D-galactosamine （D-gal）. METHODS： ALF rats were induced by Dgal, and were divided into 3 groups. Rats of group Ⅰ received 2 × 10^7 immortalized human fetal hepatocytes intrasplenically; rats of group H received 2 × 10^7 primary human fetal hepatocytes intrasplenically; rats of group Ⅲ received 1 mL normal saline intrasplenically. All rats received Cyclosporin A（CsA） at 10 mg/kg im simultaneously. Then the survival rates, liver function and liver histology of all rats were observed. RESULTS： Survival rates of rats in group Ⅰ and Ⅱ were obviously higher than that of group Ⅲ （72.2% vs 22.2%, 61.1% vs 22.2%, P 〈0.01）; the liver function and liver histology were also improved significantly as compared with those of group Ⅲ. Survival rate of group Ⅰ was close to that of groupⅡ （72.2% vs 61.1%, P〉0.05）. CONCLUSION： After human fetal hepatocyte transplantation intrasplenically, the survival rate of ALF rats intoxicated with Dgal is increased, and the liver function and histology are also improved. The immortalized hepatocytes would become the ideal cell material in the study of hepatocyte transplantation.
出处
《第四军医大学学报》
北大核心
2006年第10期879-882,共4页
Journal of the Fourth Military Medical University
关键词
永生化
胚胎肝细胞
移植
肝功能衰竭
急性
D-氨基半乳糖
immortalization
fetal hepatocytes
transplantation
liver failure, acute
D-galactosamine
