摘要
目的研究小鼠咽鼓管管旁腺腺体中β-防御素2(β-defensin 2,BD2)在急性炎症期间的表达,探讨咽鼓管管旁腺腺体在中耳防御体系中的作用。方法 20只小鼠经鼓膜途径注射3型肺炎链球菌悬液建立小鼠急性中耳炎模型,采用免疫组化技术结合图像处理技术检测急性模型1 d 组、3 d 组和7 d 组的咽鼓管管旁腺腺体中 BD2的表达。结果 1.急性模型组的咽鼓管管旁腺腺体中 BD2的表达明显高于生理盐水对照组(P<0.05);2.急性模型1、3、7 d 组 BD2的表达呈现时间依赖性的增强:7 d 组的表达显著高于前两组(P<0.05),3 d组的表达数值上略强于1 d 组,但无统计学意义(P>0.05)。结论咽鼓管管旁腺体在炎症的早期可增加 BD2的释放,并可能在中耳固有免疫防御体系中起着清除病原体的重要作用。(中国眼耳鼻喉科杂志,2006,6:143～145)
Purpose To study the expression and significance of β-defensin2（BD2） in the Eustachian paratubal glands in mice with acute otitis media （AOM）. Methods Twenty mice were used by infecting type 3 S. pneumoniae suspension through tympanic membrane, paraffin sections of Eustachian paratubal glands samples were studied with immunohistochemical technique. Results 1 ）The expressions of BD2 were greater in acute otitis media than that in controls （P〈0.05） .2）The expressions in 1 d, 3 d, 7 d of the otitis media increase in a time-dependent manner. The 7 d＇s expression were greater than the others （ P 〈 0.05）. Mthough the 3d＇ s expression were higher than the ld＇ s in value, there were no difference in statistics（ P 〉 0.05）. Conclusion It is suggested that the eustachian paratubal glands may play an important role in middle ear＇s defense system by increasing the expression of BD2 in the stage of AOM.
出处
《中国眼耳鼻喉科杂志》
2006年第3期143-145,i0001,共4页
Chinese Journal of Ophthalmology and Otorhinolaryngology
