摘要
目的:探讨耐药基因产物P-糖蛋白(P-gp)、谷胱甘肽-s-转移酶(GST-π)、拓扑异构酶Ⅱ(TopoⅡ)、胸苷酸合成酶(TS)在宫颈癌中的表达及临床意义.方法:应用免疫组织化学方法检测65例宫颈癌中P-gp、TopoⅡ、GST-π、TS的表达,并分析其与临床预后之间的关系.结果:P-gp的表达与肿瘤分化程度、淋巴结转移有关(P<0.05),与UICC分期、局部复发无关(P>0.05);TopoⅡ的表达与淋巴结转移、局部复发有关(P<0.05),与肿瘤分化程度、UICC分期无关(P>0.05);GST-π的表达与各个指标之间均有关(P<0.05);TS与淋巴结转移、UICC分期有关(P<0.05),与局部复发、肿瘤分化无关(P>0.05)。经多因素Cox比例风险模型分析显示:局部复发、淋巴结转移及TopoⅡ、GST-π的表达与宫颈癌患者术后生存率有关(P<0.05)。结论:联合检测宫颈癌中P-gp TopoⅡ、GST-π、TS对判断宫颈癌的预后十分必要;局部复发、淋巴结转移及TopoⅡ、GST-π的表达可作为宫颈癌患者独立的预后因素。
Objective： To investigate the expression of P-glycoprotein （P-gp） , glutathi- one-s-transferase-π（GST-π） ,Topo isomerase Ⅱ （ Topo Ⅱ ） and thymidylate synthase （TS） and clinical significance in cervical carcinoma. Methods： The expression of P-gp, Topo Ⅱ , GST-π, TS proteins were detected by immmunohistochemistry method in sixty-five patients with cervical carcinoma who were followed up more than ten years. The relationship between the expression of drug resistance proteins and clinical prognosis were analysed. Results：The expression of P-gp was associated with lymph node metastasis and histological grading（ P 〈 0.05）, was not associ- ated with recurrence, UICC staging （ P 〉 0.05 ）. The expression of Topo Ⅱ was associated with lymph node metastasis and recurrence （ P 〈 0.05 ）, was not associated with histological grading, UICC staging（ P 〉 0.05 ）. The expression of GST-π was associated with all parameters （ P 〈 0. 05 ）. The expression of TS was associated with lymph node metastasis and UICC grading（ P 〈 0. 05 ）, was not associated with recurrence, histological staging （ P 〉 0.05 ）. In the multivariate Cox analysis, survival rate was correlated with recurrence, lymph node metastasis, Topo Ⅱ and GST-π （ P 〈 0.05 ）. Conclusion： The detection of the drug resistance genes P-gp, TopoⅡ, GST-π and TS have an important clinical significence for the selection of chemotherapy regimen ;recurrence,lymph node metastasis and the expression of GST-π and Topo Ⅱ can be regarded as independent prognostic factors in cervical carcinoma.
出处
《现代妇产科进展》
CSCD
北大核心
2006年第4期261-264,共4页
Progress in Obstetrics and Gynecology
关键词
宫颈肿瘤
多药耐药
免疫组织化学
预后
Cervical neoplasms
Multidrug resistance
Immunohistochemistry
Prognosis
