摘要
目的:研究三七总皂苷(total sapon ins of panax notoginseng,PNS)对异丙肾上腺素所致大鼠心肌肥厚的保护作用。方法:用异丙肾上腺素(isoproterenol,ISO)5 mg.kg-1.d-1,sc,连续7 d,建立大鼠心肌肥厚模型。造模第二天开始给大鼠腹腔注射PNS 25和50 mg.kg-1.d-1,连续14 d,测定全心重量指数(HW/BW)、左心室重量指数(LVW/BW即LVI);采用试剂盒用分光光度法检测左心室心肌组织中羟脯氨酸(Hyp)和一氧化氮(NO)含量,及Na+-K+-ATP酶和Ca2+-ATP酶活性;用放免分析法检测左心室心肌组织中血管紧张素Ⅱ(AngⅡ)含量。结果:ISO模型组大鼠的HW/BW、LVI明显升高;左心室Hyp、AngⅡ含量增高,NO水平下降,Na+-K+-ATP酶和Ca2+-ATP酶活性降低。PNS能明显提高心肌组织NO水平及Na+-K+-ATP酶和Ca2+-ATP酶活性,降低胶原的含量,抑制AngⅡ产生,使HW/BW及LVI下降,抑制心肌肥厚。结论:PNS对ISO所致大鼠心肌肥厚具有保护作用。
Objective： To investigate the protective effects of total saponins of panax notoginseng （PNS） on myocardial hypertrophy induced by isoproterenol （ISO） in rats. Methods： Myocardial hypertrophy model of rats were induced by injection of ISO （5mg ·kg^-1 · d^-1 ,so） for 7d, from day 2, the rat were administered with PNS 25 and 50mg · kg^-1 · d^-1 , ip for 14d,the control and ISO model group were received saline injection. Then, the heart - weight （HW） ,left ventrieuiar weight （ LVW）, the ratio of HW/BW and LVW/ BW （LVI） were measured; the content of hydroxyproline and angiotensin （AnglI） , the level of nitric oxide（NO） and the activity of Na^＋ -K^＋ -ATPase Ca^2＋ -ATPase in left ventricle were determined by speetrophotometry and radioimmunoassay, respectively. Resuits： In ISO model group , HW/BW ,LVI and the content of hydroxyproline and AngII in left ventricle markedly increased; the level of NO and the activity of Na^＋ - K^ ＋ - ATPase, Ca^2＋ - ATPase in the left ventricle were decreased. Treatment with PNS significantly reduced the HW/BW,LVI, decreased the AngⅡ and the hydroxyproline content, increased the lewel of NO and the activity of Na^＋ -K^ ＋ - ATPase Ca^2 ＋ - ATPase.
出处
《赣南医学院学报》
2006年第2期161-164,共4页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
关键词
三七总皂苷
异丙肾上腺素
心肌肥厚
大鼠
PNS was shown to prevent remodeling of myocardial hypertrophy induced by ISO in rats.
