摘要
本文利用超声波对两种乳酸杆菌细胞进行破碎,以提取细胞中的亚油酸异构酶,并就超声波破碎条件对亚油酸异构酶释放的影响进行了研究。通过单因素试验和正交试验得出嗜酸乳杆菌的最佳超声破碎条件为:间歇破碎次数80次(每次破碎3s,中间间隔4s),破碎菌液体积50ml,菌悬液浓度20g/60ml,破碎时的抽提缓冲液最佳pH值4.0,NaCl浓度0.2mol/L;保加利亚乳杆菌的最佳超声破碎条件为:间歇破碎次数80次(每次破碎3s,中间间隔4s),破碎菌液体积60ml,菌悬液浓度15g/50ml,破碎时的抽提缓冲液最佳pH值4.8,NaCl浓度0.3mol/L。
Ultrasonic wave was used in this paper to disrupt the cells of two Lactobacillus species so as to extract linoleate isomerase from the cells, and the effects of the disruption conditions to release linoleate isomerase were studied. After single-factor experiment and orthogonal experiment, the optimum disruption conditions for Lactobacillus acidophilus were： intermittent ultrasonic treatment 80 times （time for each treatment was 3s, with a 4s interval）, volume of the treated cell suspension 50ml, concentration of cell suspension 20g/60ml, pH value of the extraction buffer 4.0, and concentration of NaCl2.0mol/L; while the optimm conditions for Lactobacillus bulgaricus were： intermittent ultrasonic treatment 80 times （time for each treatment was 3s, with a 4s interval）, volume of the treated cell suspension 60ml, concentration of cell suspension 15g/50ml, pH value of the extraction buffer 4.8, and concentration of NaCl3.0mol/L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第5期36-40,共5页
Food Science
基金
河南省杰出青年科学基金项目(04120001800)
河南省教育厅自然科学基础研究项目(2004180010)
河南省高校杰出科研人才创新工程项目(2006KYCX008)
关键词
嗜酸乳杆菌
保加利亚乳杆菌
亚油酸异构酶
超声波破碎
条件
Lactobacillus acidophilus
Lactobacillus bulgaricus
linoleate isomerase
ultrasonic wave disruption
condition
