摘要
目的:1,25-二羟维生素D3[1,25-(OH)2D3]对连续多次小剂量链脲菌素(the mu ltip le low dose streptozotoc in,MLDS)诱导的自身免疫性糖尿病小鼠的预防作用及其机制初探。方法:小鼠分为三组。正常对照组:连续5天腹腔注射与糖尿病组等容量柠檬酸盐缓冲液;糖尿病组:连续5天腹腔注射STZ(40mg.kg-1),以血糖水平持续高于16.7mmol/L为成模标准;预防组:先隔日腹腔注射1,25-(OH)2D3(5μg.kg-1),共15次,然后再连续5天腹腔注射STZ(40mg.kg-1)。实验结束后各组动物处死收集血清并采集胰腺检测诱导型一氧化氮合酶(iNOS)活性及血清胰岛素水平。结果:MLDS诱发的自身免疫性糖尿病模型在第四周基本建成。MLDS使小鼠血糖、血清及胰腺iNOS活性升高,血清胰岛素水平下调;预防组小鼠注射STZ前给予1,25-(OH)2D3有明显降血糖和上调血清胰岛素水平作用,同时抑制血清及胰腺iNOS活性,与糖尿病组比较有显著性差异(P<0.05)。结论:1,25-(OH)2D3可有效预防MLDS诱导的自身免疫性糖尿病的发生。该效应可能与1,25-(OH)2D3抑制iNOS活性有关。
Objective：To investigate the preventive effect of 1,25-dihydroxyvitamin Ds on type 1 diabetes in streptozotocin-indueed KM mice. Methods： Normal control group： KM mice were treated with citrate buffer i. p. for 5 consecutive day. Diabetes group： After streptozotocin （40mg · kg^-1 ） i.p. was given for 5 consecutive days. The blood glucose level was monitored over the following 6 weeks, the mice were considered diabetic when sequential blood glucose level was equal to or above 16.7mmol/L. 1,25-dihydroxyvitamin Ds prevention group： KM mice were pretreated with 1,25-dihydroxyvitamin D3 （5μg · kg^-1） i. p. every other days, and then streptozotcin （40mg · kg^-1） was given intraperitoneal for 5 consecutive days. Blood glucose level was monitored using a one-touch blood glucose meter. Radioimmunnity analysis was used to detect the serum insulin level. Serum and pancreas induced nitric oxide synthase （iNOS） were measured using the commercially available kits. Results： MLDS-induced model of type 1 diabetes was finished in the fourth weeks. Blood glucose level, serum and pancreas iNOS level increased. The serum insulin level decreased. Diabetes incidence of KM mice can be prevented when 1,25-dihydroxyvitamin D3 administrated before streptozotocin treatment. The blood glucose, serum and pancreas iNOS level decresed and the serum insulin level increased in the 1,25-dihydroxyvitamin D3 prevented mice. Conclusion： These results suggest that 1,25-dihydroxyvitamin D3 pre-treatment could reduce the incidence of autoimmune diabetes in mice model by suppressing iNOS.
出处
《四川生理科学杂志》
2006年第1期26-28,共3页
Sichuan Journal of Physiological Sciences
