摘要
在已知萝卜D染色体附加系对线虫具有抗性和萝卜分子图谱的7号连锁群定位有抗线虫基因Hs1Raph的基础上,利用(dp)RAPD分子标记技术,以甘蓝型油菜异源萝卜附加系为材料,筛选到90个萝卜D染色体的特异标记,选择4个在萝卜构图亲本间表现多态性的D染色体标记进行F2群体(包括245个单株)分离检测,并与构图用的505个AFLP和RAPD标记一起用Join-map3.0进行连锁定位分析。结果萝卜D染色体标记全部定位于7号连锁群,并且7号连锁群上只有D染色体的特异标记,说明萝卜7号连锁群就是D染色体在分子水平的反映。证实了定位有抗线虫基因Hs1Raph的7号连锁群对应于具线虫抗性效应的萝卜D染色体。实验结果对研究萝卜D染色体控制的农艺性状或抗逆性以及萝卜抗线虫育种和将萝卜抗线虫基因转入其它芸薹属作物的研究都将会起到重要的指导作用。
Chromosome D of Raphanus sativus has been determined to be the nematode resistance-bearing,and linkage group 7 of Raphanus sativus has been located the gene HsI^Raph controlling resistance to nematode.On this basis,this work was carried out to research the relation of chromosome D and linkage group7 to see whether linkage group 7 is essentially the map of chromosomes D on DNA level.90 markers assigned to chromosome D were screened from rape-radish addition lines by using(dp)RAPD technology.4 polymorphic assigned markers were further mapped in F2 population comprising of 245 individuals,and then were analyzed by software join-map 3.0 together with 505 AFLP and RAPD markers,which has been used to construct radish molecular map.As a result,all of chromosome D assigned markers were located into linkage group 7,and in linkage group 7,there were no markers assigned to any other chromosomes of radish.It could be proven that linkage group 7 carrying the nematode resistance gene Hsl^Raph is identical with chromosome D bearing the resistance to nematode.The result will be useful to search for inter-genomic recombination in a program intended to introgress nematode resistance from radish to other Brassica crops using chromosome additions,as well as useful to map further resistance traits in radish.
作者
丁云花
Budahn Holger
Peterka Herbert
Ding Yunhua;Holger Budahn;Herbert Peterka(Beijing Vegetable Research Center,Beijing 100089;Federal Centre for Breeding Research on Cultivated Plants,Quedlinburg D–06484,Germany)
出处
《中国农学通报》
CSCD
2006年第5期68-71,共4页
Chinese Agricultural Science Bulletin
基金
农业部中德农业合作项目"蔬菜分子标记辅助育种研究"(2001/2002)。
关键词
萝卜
附加系
D染色体
连锁群
Raphanus sativus
Addition lines
Chromosome D
Linkage group
