摘要
基于对磺酸苯亚甲基硫代若丹宁（SBDTR）与汞的显色反应和C18固相萃取小柱对显色络合物的固相萃取，建立了一种测定汞的新方法。在pH3．8的醋酸-醋酸钠缓冲介质中，乳化剂-OP存在下。SBDTR与汞反应生成2：1稳定的红色络合物。该络合物可被C18固相萃取小柱萃取富集，小柱上富集的络合物用氮-氮二甲基甲酰氨（DMF）洗脱后富集倍数可达50倍，洗脱液用分光光度法测定，在测定液中，体系最大吸收波长λmax-545nm，摩尔吸光系数ε=1．22×10^5L·mol^-1·cm^-1。汞含量在0．01～3．0μg·ml^-1范围内符合比耳定律，本方法可用于食品和水样中汞含量的测定。
A new method for the determination of mercury based on the rapid reaction of mercury （ Ⅱ ） with p-sulfobenzylidenethiorhodanine （ SBDTR ） and the solid phase extraction of the colored chelate with C18 disks was developed. In the presence of pH = 3.8 sodium acetate-acetic acid buffer solution and Emulsifier-OP medium, SBDTR reacts with mercury （ Ⅱ ） to form a red chelate of a molar ratio 1 ： 2 （mercury to SBDTR）. This chelate is enriched by the solid phase extraction with C18 disks and the chelate is eluted from the disks with dimethyl formamide （DMF）. The enrichment factor of 50 is achieved. In the DMF medium, the molar absorptivity of the chelate is 1.22 × 10^5 L·mol^-1·cm^-1 at 545 nm. Beer＇s law is obeyed in the range of 0.01 -3 μg·ml^-1 in the measured solution. The relative standard deviation for eleven replicates sample of 0.01 μg·ml^-1 level is 1.65%. This method has been applied to the determination of mercury in food and water with good results.
出处
《稀有金属》
EI
CAS
CSCD
北大核心
2006年第2期168-171,共4页
Chinese Journal of Rare Metals
关键词
对磺酸苯亚甲基硫代若丹宁
汞
固相萃取光度法
mercury
solid phase extraction spectrophotometry
p-sulfobenzylidenethiorhodanine
