摘要
目的探讨长期氡及其子体吸入对职业氡暴露人群与大鼠的氧化损伤,寻找早期效应标志物。方法用固体核径迹法测定作业场所中氡浓度,利用1 Bq/m3=1.26×10-3WLM(工作水平月)的转换系数算出单位氡浓度氡子体年暴露量。职业氡暴露人群按照氡子体累积暴露剂量分为2,26和106 WLM即3个低、中、高剂量组。雄性Wistar大鼠15只,随机分成3组,低剂量组暴露于普通环境中,中、高剂量组整体暴露于多功能生态氡室,氡子体累积暴露剂量分别达1,27和105工作水平月。检测各组人群和各组大鼠外周血的超氧化物岐化酶(SOD)活力、丙二醛(MDA)含量。结果在低、中、高剂量职业氡暴露人群外周血中,SOD活力分别为(117.34±18.07)U/ml、(83.32±19.79)U/ml和(60.37±21.40)U/ml,MDA含量分别为(1.85±1.12)nmol/ml、(2.68±1.36)nmol/ml和(3.35±1.61)nmol/ml。随着氡子体累积暴露剂量的增加,SOD活力逐渐降低(P<0.05),MDA含量逐渐升高(P<0.05)。在低、中、高剂量吸入氡染毒大鼠外周血中,SOD活力分别为(1 393.40±88.30)U/ml、(1 198.60±132.44)U/ml和(1 098.60±50.90)U/ml,MDA含量分别为(0.53±0.13)nmol/ml、(1.89±0.13)nmol/ml和(2.18±0.18)nmol/ml。随着氡子体累积暴露剂量的增加,SOD活力逐渐降低(P<0.05),MDA含量逐渐升高(P<0.05)。结论SOD与MDA可作为氡及子体吸入引起氧化损伤的早期效应标志物。
Objective To study the level of oxidative stress impairment in peripheral blood of occupational population and rats exposed to radon and its daughters and to explore for the early biomarkers of effects. Methods The concentration of radon was monitored by the method of the solid nuclear traces and was transferred to the concentration of its daughters according to 1 Bq/m^3 = 1.26×10^-3 WLM. Fifteen male Wistar rats were randomly divided into three groups, one group exposed to common environment, the other two groups exposed to multi-functional ecology radon cabinet. Occupational population exposed to radon and its daughters were divided into three groups according to cumulative doses, 2 WLM （low dose）, 26 WLM （medium dose） and 106 WLM （high dose）. The cumulative dose of rats was 1 WLM （low dose）, 27 WLM （median dose） and 105 WLM （ high dose） respectively. Activity of SOD （superoxide dismutase） and content of MDA （malondialdehyde） in peripheral blood were detected. Results The activities of SOD in peripheral blood of low, medium and high doses groups were （117. 34 ±18. 07 ） , （83.32±19.79） and （60.37±21.40） U/mlfor human being, （1 393.40±88.30）, （1 198.60±132.44） and （1 098.60±50.90） U/ml for rats, respectively. The contents of MDA in peripheral blood of low, medium and high doses groups were （1.85±1.12）, （2. 68±1.36） and （3.35±1.61） nmol/ml for human being, （0.53±0.13）, （1.89± 0.13） and （2.18±0.18） nmol/ml for rats respectively. The activities of SOD decreased significantly and contents of MDA increased with the cumulative doses （P〈0.05）. Conclusions SOD and MDA could be the candidates of the early biomarkers of oxidative stress impairments induced by radon and its daughters.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2006年第3期143-146,共4页
Industrial Health and Occupational Diseases
基金
国家自然科学基金项目(30371226)
江苏省重点实验室开放课题基金(KJS01068)
