摘要
以91株‘东光’和‘富士’的正反交F1代群体为试材,测定了成熟果实可滴定酸含量和果实不同发育阶段苹果酸含量的变化,从表型上分析了苹果酸的遗传规律。利用140对共显性遗传的SSR引物对高酸和低酸个体群体分离分析,筛选到一个与果实酸/低酸性状连锁的标记SDY085,遗传距离为8.89cM,表型分析和标记分析都表明苹果果实酸/低酸性状由一对主效基因Ma/ma控制,并且Ma对ma为完全显性,而酸个体中苹果酸的连续分布则是加性多基因作用的结果。
It is necessary for apple genomic research and breeding to find out malic acid genetic characteristics. In this research, SSR marker linked to acid/low-acid trait in apple fruit was identified from 140 SSR primer pairs, materialed by 91 F1 population hybrid from the intra-specific cross between apple cultivar ‘Dongguang＇ and‘Fuji＇. Of 140 SSR primer pairs, only primer SDY085 produced a polymorphic band linked to acid trait, and the linkage distance was 8.89 cM. Also, the titrated acid and malic acid in different developmental stages were determined. The SSR marker analysis, coupled with the change of the total acid and malic acid contents, revealed that acid/low-acid trait was governed by a major gene and acid trait was complete dominant.
出处
《园艺学报》
CAS
CSCD
北大核心
2006年第2期244-248,共5页
Acta Horticulturae Sinica
基金
山东省2003良种产业化项目(32271)
关键词
苹果
酸/低酸性状
SSR标记
苹果酸
Apple
Malus × domestica Borkh.
Acid/low-acid trait
SSR marker
Malic acid
