摘要
目的:研究清胰汤(QYT)对大鼠急性出血坏死性胰腺炎(AHNP)肺损伤的治疗机理。方法:Wistar大鼠经胆胰管逆行注射5%牛磺胆酸钠(1mL/kg,0.1mL/min)诱发AHNP;QYT组在造模后30min灌胃QYT(10mL/kg);善宁组在造模后30min皮下注射善宁(50μg/kg)。每组又分别随机分为36、、12h三组(每组8只),其余12只用于观察24小时死亡率。于不同时间点测定血清淀粉酶(AMY)、脂肪酶(LIP)、磷脂酶A2(PLA2)、肿瘤坏死因子(TNFα);测定肺组织髓过氧化物酶(MPO)、PLA2、TNFα;提取肺泡灌洗液巨噬细胞核蛋白,ELISA法检测核因子(NF-κBP65)表达;对肺、胰腺组织行病理检查。结果:QYT明显降低AHNP大鼠死亡率;减轻肺、胰腺病理损伤;降低血清、肺组织PLA2、TNFα水平;显著降低肺泡巨噬细胞NF-κB活性。结论:清胰汤可通过其多途径多靶点作用,抑制PLA2活性,抑制肺内炎症相关细胞NF-κB活化,下调多种细胞因子表达,减轻肺损害,降低死亡率。
Objective： To investigate the therapeutic mechanisms of Qing Yi decoction on acute lung injury induced by acute hemorrhagic necrotizing pancreatitis in rats. Methods： AHNP model was induced by intraductul administration of 5 % sodium taurocholate in Wistar rat. Qing Yi decoction was administered orally 30 minutes after model induction. Sandostain （50μg/kg） was administrated by subcutaneous injection after model induction. The other animals were used to observe the 24hour-mortality. Animals were killed on various hours after model induction （3, 6, 12h）. Bronchoalveolar lavage （BAL） macrophages NF-κB activation was determined by ELISA method. Pulmonary TNF, PLA2, myeloperoxidase（MPO）were examined. The Levels of Amylase, Lipase, PLA2 of serum were determined also. Morphologic changes of lung and pancreas were observed under light microscopy. Results： In Qing Yi decoction group, the mortality was significantly reduced; Lung and pancreas injury was markedly reduced; Qing Yi decoction significantly attenuated NF-κB activation of BAL macrophages. PLA2, TNFα levels of serum and lung tissue were decreased significantly. Conclusions： Qing Yi decoction has protective effects on AHNP-ALI through multiple pathways, among these, the inhibitory effect on PLA2 activity is an important one. Qing Yi decoction can attenuate the ＂PLA2-NFκB-cytokine cascade＂ markedly.
出处
《中国实验方剂学杂志》
CAS
2006年第5期43-46,共4页
Chinese Journal of Experimental Traditional Medical Formulae
